Letters in Biotechnology 2015 Issue 4 Co-Expression of Cas9 and sgRNA in One Plasmid Improves Tar-geting Efficiency at the AAVS1 Locus

Co-Expression of Cas9 and sgRNA in One Plasmid Improves Tar-geting Efficiency at the AAVS1 Locus
YU Jun-Yao;,LI Xiao-Lan;,ZHANG Jian-Ping;,CHENG Tao;,ZHANG Xiao-Bing;

..............page:449-453

Anti-Enterovirus 71 and Mechanism of Polysaccharides Extracted from Selaginella tamariscina
XIA Yan-Ping;,MA Teng;,QI Xiang-Yun;,LI Qing-Jun;,WANG Sheng-Qi;,YANG Jing;

..............page:454-459

Selection of Epitope of F1 Antigen of Yersinia pestis by Phage Dis-play Peptide Library
YIN Ke-Xin;,CHI Xiang-Yang;,REN Jun;,FANG Ting;,LIU Shu-Ling;,LIU Ju;,YANG Xiu-Xu;,LI Jian-Min;,YU Chang-Ming;

..............page:460-465

Effects of the Patterns of Helicobacter pylori CagA EPIYA Motifs on AGF Cell Morphology and IL-8 Secretion Levels
LIU Lu;,WANG Yan-Chun;,TAO Hao-Xia;,YUAN Sheng-Ling;,WANG Ling-Chun;,LIU Chun-Jie;

..............page:466-470

ren ti wei sheng wu zu
hua xue gong ye chu ban she chu ban ;

..............page:470-470

Reconstruction, Prokaryotic Expression, Purification of Recombinant F1-V Mutant from Yersinia pestis
FANG Ting;,YIN Ke-Xin;,REN Jun;,ZHANG Xiao-Peng;,YU Rui;,SONG Xiao-Hong;,YANG Xiu-Xu;,YU Chang-Ming;

..............page:471-476

Expression of Recombinant Human IL-7 in CHO Cell and its Ef-fect on Promoting PBMC Proliferation
LI Jin-Song;,MA Fen-Lian;,ZHANG Qian;,ZHENG Li-Shu;

..............page:477-480

Expression and Function Identify of Leptospirosis Laminin-Binding Protein Las20
WEI Ying;,YUAN Sheng-Ling;,YAO Xue-Jing;,TAO Hao-Xia;,WANG Ling-Chun;,LIU Chun-Jie;,TIAN Wei;,WANG Yan-Chun;

..............page:481-484

Construction and Expression Identification of the GFP-LC3B Eu-karyotic Expression Vector
ZHU Jie;,DING Li-Hua;,LIU Jie;,HE Rong-Hua;,ZHANG Ya-Nan;,CHEN Zhi-Da;,LUO Xiao-Li;,YE Qi-Nong;,LYU Zhao-Hui;

..............page:485-488

jian ming gan xi bao sheng wu xue
hua xue gong ye chu ban she chu ban ;

..............page:488-488

Construction of Flag-Tagged Human NKX2-3 Eukaryotic Expres-sion Vector and its Role on Tumor Cell Growth
FENG Ying-Ying;,GUO Jing;,LIU Jia-Hong;,XU Xiao-Jie;,ZHANG Yun-Jing;,CHEN Yun-Ping;,YE Qi-Nong;,ZHAO Ke;

..............page:489-492

Cloning and Expression of a Dominant Epitope Fragment of Rabies Glycoprotein and its Application in Neutralizing Antibody Detection
QUE Hai-Ping;,GUO Zai-Qing;,HUANG Yun-Zhou;,GUO Lan-Qin;,ZHU Cui-Xia;,WANG Guo-Hua;,SONG Xiao-Guo;,ZHANG He-Qiu;,FENG Xiao-Yan;

..............page:493-496

Expression and Purification of Human N-Ras Protein with Fused GST Protein Expression System
ZHANG Yun-Jing;,FENG Ying-Ying;,XU Xiao-Jie;,LIANG Ying-Chun;,ZHANG Li;,WANG Tao;,LI Ling;,GUO Jing;,JI Bei-Bei;,ZHANG Rong;,YE Qi-Nong;

..............page:497-500

Recombinant Chlamydial ChxR Has Extensive DNA Binding Activi-ties to Upstream Sequences of Plasmid-Regulated Genes in Vitro
LI Peng;,YU Yong-Hui;,WANG Tao;,HE Jun;,ZHU Hong;,DUAN Qing;,SONG Li-Hua;

..............page:501-504

Screening and Identification of Antigen Epitope of hB7-H4 Recog-nized by Monoclonal Antibody with 12 phage Display Peptide Li-brary
LI Xiao-Ju;,GAO Yuan;,ZHANG Wang-Qian;,ZHANG Cun;

..............page:505-509

zhi wu zu pei kuai fan di 1 juan bei jing yuan zhu di 3 ban
hua xue gong ye chu ban she chu ban ;

..............page:509-509

A Lentiviral Vector Used for Novel Short Hairpin RNA Expression and its Application for Anti-Hepatitis B Virus
ZHANG Ting-Yu;,ZHANG Xiu-Juan;,MAO Ying-Ying;,HU Wei;,WANG Xue-Jun;,WANG Sheng-Qi;

..............page:510-514

Toxicity Study of Recombinant Human B7- H1 IgV Vaccine in BALB/c Mice
CAO Zheng-Cong;,ZHANG Cun;,WU Shou-Zhen;,ZHANG Wang-Qian;,ZHANG Ying-Qi;,ZHANG Wei;

..............page:515-518

Effect of Brucea javanica Fruit Oil Emulsion Combination with Cis-platin on the Growth of Human Ovarian Caner SKOV3 Cells Nude Mouse Orthotopic Transplantation Tumor
ZHAO Nan;,HAN Feng-Juan;,WANG Gui-Yuan;,LI Yu-Hua;

..............page:519-523

fen zi yi chuan xue yuan li shang ce
hua xue gong ye chu ban she chu ban ;

..............page:523-523

Flutide in vivo Protection Against FM1 of H1N1 Influenza Virus
LI Qing-Jun;,ZHANG Bo;,XIA Yan-Ping;,WANG Sheng-Qi;,YANG Jing;

..............page:524-526

Function Expression of HCN Channel in Xenopus Oocytes
SUN Yan;,ZHANG Shu-Zhuo;,ZHUO Ren-Gong;,LIU Xiao-Yan;,MA Xiao-Yun;,WEI Xiao-Li;,LIU Ying;,ZHENG Jian-Quan;

..............page:527-530

Study of Mutation of BRCA1 and BRCA2 Gene Among Familial Breast Cancer Patients and Healthy Genetic Groups at High Risk
LI Chen;,JIN Yan-Wen;,LIU Xiao-Jing;,JIN Shu-Juan;,HAN Xiao-Wei;,PANG Han-Min;,HAO Xiao-Peng;,CAO Cheng;,HUANG Yan;

..............page:531-534

Study on the Regulation Fuction of Small RNAs on Cellulase Ex-pression in Trichoderma reesei
LIANG Zhi-Wei;,WANG Juan;,TIAN Sheng-Li;,WANG Li-Yan;,LIU Gang;

..............page:535-540

Association of Leaf Morphological Characteristics and Glycyrrhizic Acid Contents in Glycyrrhiza uralensis with Different Copy Number Variations of Functional Genes
WANG Li-Qiang;,YANG Rui;,YUAN Bo-Chuan;,LIU Chun-Sheng;,LIU Ying;

..............page:541-545

Effects of Different Factors on the Growth and Saikosaponins Con-tent of Bupleurum chinense Hairy Root
SUN Jing;,GAO Ke;,WANG Ling;,WU Su-Rui;,YANG Hong-Yi;,SUI Chun;

..............page:546-550

Comparative Study of Bacterial Nucleic Extraction Method on 16S rDNA Metagenomic Sequencing
WANG Wei;,WANG Yu-Zhuo;,SHU Peng;,MI Zhi-Qiang;,AN Xiao-Ping;,PEI Guang-Qian;,LIU Wen-Li;,YUAN Wen-Jun;,SHI Tao-Xing;,TONG Yi-Gang;

..............page:551-555

Development and Verification of a Novel in vitro Assay for Determi-nation of Biological Activity of Human Follicle Stimulating Hormone
JIA Hui;,QI Lian-Quan;,LIANG Yan;,CHANG Cui-Yun;,WANG Xin;,XU Jun-Jie;,CHEN Wei;

..............page:556-561

Survey of Bone Metabolism in Fighter Pilot
WANG Ping;,ZHOU Wei-Guo;,LI Min;,PAN Shi-Xin;,SHI Yu-Ling;,BAN Wu;

..............page:562-564

Complementary Resistance Strategy for Direct Cloning of Large DNA Fragments from Chromosome
YANG Yan;,ZHU Ying;,ZHU Mei-Qin;,WEI Yan-Long;,NI Meng-Xiang;,FANG Hong-Qing;

..............page:565-569

Research Progress of Bacterial Signaling Molecule of Antibiotics Re-sistance Mechanism
YI Sheng-Jie;,XIE Jing;,QIU Shao-Fu;,SONG Hong-Bin;

..............page:570-573,578

Progress in the Application of Metabonomics Technology in Respira-tory Disease Research
DU Xiang-Bo;,ZHOU Ning;,SUN Bo;,ZHANG Qi;,XU Mei-Feng;,YAN Xian-Zhong;

..............page:574-578

Glucagon-Like Peptide-1 and Pancreatic β Cells
SONG Xiao-Yan;,HE Wen-Jun;,SUN Xiao-Ya;,HAO Hao-Jie;,HAN Wei-Dong;,MU Yi-Ming;

..............page:579-582

Expression of Estrogen Receptor in Brain and Ischemia-Reperfusion Injury
ZHANG Zhi-Chun;,ZOU Ping;,HUANG Liang;,ZOU Wei;

..............page:583-586

Advances in Active Constituents of Traditional Chinese Medicines for Treating Alzheimer's Disease
MENG Ye;,ZHANG Qing-Lin;

..............page:587-590

Surface Enhanced Raman Spectroscopy and its Application in the Detection of Foodborne Pathogens
CAO Wei-Li;,WANG Chong-Wen;,XIAO Rui;,WANG Sheng-Qi;

..............page:591-594

zhi zuo zhe
sheng wu ji shu tong xun bian ji bu ;

..............page:595-595