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Chinese Journal of Biotechnology
1000-3061
2009 Issue 8
Expression,purification and characterization of bacteriophage lysin of Streptococcus in Escherichia coli
Weiqing Chen1;Xiaofeng Wang2;Pu Wang2;Deyong Zhang1;Hong Chen1;Wei Ke1;Yin Lu1;and Jianfen Zhang1 1 College of Biological and Environmental Engineering;Zhejiang Shuren University;Hangzhou 310015;China 2 College of Pharmaceutical Science;Zhejiang University of Technology;Hangzhou 310032;China
..............page:1267-1272
Recombinant expression of the fusion antigen based on Treponema pallidum TpN17 and TpN47 epitope peptides and establishment and application of the associated ELISA
Aihua Sun 1;Xingli Fan 1;Xiangdi Shen 1;Renxian Tang 2;and Jie Yan 3 1 Department of Basic Medicine;Zhejiang Medical College;Hangzhou 310053;China 2 Department of Pathogenic Microbiology;Xuzhou Medical College;Xuzhou 221002;China 3 Department of Medical Microbiology and Parasitology;College of Medicine;Zhejiang University;Hangzhou 310058;China
..............page:1187-1194
A novel screening system for Pseudomonas aeruginosa quorum sensing inhibitors
Weijie Sun;Yuan Wang;Lixin Shen;and Kangmin Duan Key Laboratory of Resources Biology and Biotechnology in Western China;Ministry of Education;College of Life Sciences;Northwest University;Xi’an 710069;China
..............page:1173-1179
Rabdocoetsin B,a diterpenoid isolated from Isodon coetsa,is a potential proteasome inhibitor and induced apoptosis of t(8;21) leukemia cells
Tingting Feng1;2;5;Jianxin Pu3;Zheng Hu1;Dapeng Liu1;Handong Sun3;and Guangbiao Zhou1;2;4 1 Guangzhou Institute of Biomedicine and Health;Chinese Academy of Sciences;Guangzhou 510663;China2 School of Life Science;University of Science and Technology of China;Hefei 230027;China 3 Kunming Institute of Botany;Chinese Academy of Sciences;Kunming 650204;China 4 State Key Laboratory of Biomembrane and Membrane Biotechnology;Institute of Zoology;Chinese Academy of Sciences;Beijing 100101;China 5 College of Basic Medical Sciences;Anhui Medical University;Hefei 230032;China
..............page:1218-1224
Cloning,expression and characterization of HSP gene from Eimeria tenella
Yan Yan;Hongyu Han;Bing Huang;Qiping Zhao;Hui Dong;Lianlian Jiang;Yujian Li;Yujuan Fan;and Qian Yao Key Laboratory of Animal Parasitology of Ministry of Agriculture;Shanghai Veterinary Research Institute;Chinese Academy of Agricultural Sciences;Shanghai 200241;China
..............page:1121-1129
Real-time RT-PCR based on DNA subtraction for absolute quantification of gene expression in engineered lactic acid bacteria
Rui Shi;Fei Liu;Guicheng Huo;and Lijie Yang Key Laboratory of Dairy Science;Ministry of Education;Northeast Agricultural University;Harbin 150030;China
..............page:1240-1246
Purification of glutathione-S-transferase fusion protein by glutathione coupled magnetic particles
Jingjing Zhu 1;Liu Yang 2;Lei Yang 1;Chao Chen 1;2;and Yali Cui 1;2 1 School of Life Sciences;Northwest University;Xi’an 710069;China 2 National Engineering Research Center of Miniaturized Detection Systems;Xi’an 710069;China
..............page:1254-1260
Identification and application of yeast histone acetyltransferases Elp3 polyclonal antibody
Fen Li;Shujuan Tian;Shuai Zhang;Yan Kong;and Yanfang Wang College of Life Sciences;Henan Normal University;Xinxiang 453007;China
..............page:1261-1266
Heterologous expression of particulate methane monooxygenase in different host cells
Bing Han1*;Tao Su2*;Cheng Yang1;Hao Jiang1;Hao Wu1;Chong Zhang1;Xin Li2;and Xinhui Xing1 1 Institute of Biochemical Engineering;Department of Chemical Engineering;Tsinghua University;Beijing 100084;China 2 Graduate School of Chinese Academy of Agricultural Sciences;Beijing 100081;China
..............page:1151-1159
High expression of antimicrobial peptide Cecropin AD in Escherichia coli by fussion with EDDIE
Zhen Zhang1*;Tao Ke2*;Yuling Zhou1;Xiangdong Ma1;and Lixin Ma1 1 College of Life Science;Hubei University;Wuhan 430062;China 2 College of Life Science and Technology;Nanyang Normal University;Nanyang 473061;China
..............page:1247-1253
Isolation and identification of a human single chain Fv antibody against amyloid-beta 1-42 soluble oligomers from a human phage display library
Fuxiang Bao1;2;Jinsheng He1;Guifang Cao2;Fan Yin1;Xin Wang1;Siyuan Pang1;Ying Zhang1;and Tao Hong1 1 College of Life Sciences & Bioengineering;Beijing Jiaotong University;Beijing 100044;China 2 College of Animal Science and Veterinary Medicine;Inner Mongolia Agricultural University;Huhhot 010018;China
..............page:1195-1203
Establishment of xenograft mouse models to study human lung cancer by using in vivo imaging system
Haiyan Zhang1;2;Yan Li1;Zhe Wang1;and Biliang Zhang1;2 1 State Key Laboratory for Respiratory Diseases;Guangzhou Institute of Biomedicine and Health;Chinese Academy of Sciences;Guangzhou 510663;China 2 University of Science and Technology of China;Hefei 230026;China
..............page:1204-1210
Impact of exogenous paraquat on enzyme exudation and biochemical changes of lignin degradation fungi
Yunchen Zhao1;2;Jianlong Li1;Yuru Chen3;Haixia Huang1;and Zui Yu1 1 State Key Laboratory of Pharmaceutical Biotechnology;School of Life Science;Nanjing University;Nanjing 210093;China 2 Department of Agronomy;Hexi University;Zhangye 734000;China 3 School of Life Science;Nanjing Normal University;Nanjing 210046;China
..............page:1144-1150
Cloning and characterization of porcine NOBOX gene
Guiqiang Li;Meili Li;Wei Fang;Wei Wang;Yu He;Xiaoguang Song;and Yinxue Xu College of Animal Science and Technology;Nanjing Agricultural University;Nanjing 210095;China
..............page:1130-1137
Immunogenicity of Staphylococcus aureus recombinant clumping factor A
Hao Feng 1;Lefeng Liu 1;Jiaqi Chi 2;Ning Wang 2;Runting Li 1;Chunyu Tong 1;Jinzhu Ma 1;Zhanbo Zhu 2;and Yudong Cui 1;2 1 School of Life Science and Technology;Heilongjiang Bayi Agricultural University;Daqing 163319;China 2 School of Animal Science and Technology;Heilongjiang Bayi Agricultural University;Daqing 163319;China
..............page:1180-1186
Patterning different cells based on microfluidics and self-assembled monolayers
Jianzhang Wu1;2;Jianbo Shao1;2;Yunhuan Zheng1;2;Qinghui Jin1;and Jianlong Zhao1 1 Institute of Microsystem and Information Technology;Chinese Academy of Science;Shanghai 200050;China 2 Graduate University of Chinese Academy of Science;Beijing 100049;China
..............page:1234-1239
Recombinant expression of bovine chymosin in Pichia pastoris
Li Zhang;Yuanyuan Jiang;Jian Zhang;and Zhennai Yang Center of Agro-food Technology;Northeast Agricultural Research Center of China;Changchun 130033;China
..............page:1160-1165
Production of cloned goats by transfer of nuclei from transfected caprine mammary gland epithelial cells
Yuguo Yuan;Guoliang Ding;Liyou An;Junhui Zhao;Yujuan Cao;Mingxing Miao;and Yong Cheng Jiangsu Provincial Research Center for Animal Transgenesis and Biopharming;College of Veterinary Medicine;Yangzhou University;Yangzhou 225009;China
..............page:1138-1143
Recombinant human gapM1 expressed in Pichia pastoris and its anti-diabetic effect
Xiang Mei 1;Renqian Du 1;Xi Li 1;2;Haiyan Huang 1;2;Min Yu 2;and Qiqun Tang 1;2 1 Institutes of Biomedical Sciences;Fudan University;Shanghai 200032;China 2 Key Laboratory of Molecular Medicine;Ministry of Education;Fudan University;Shanghai 200032;China
..............page:1211-1217
Soluble expression,purification and immunoreactive identification of mouse zona pellucida 3 fusion protein
Meiyu Sun;Zhenghai Ma;Yongxin Li;Tao LüKaixu Chen;and Fuchun Zhang Key Laboratory of Biological Resources and Genetic Engineering;College of Life Science and Technology;Xinjiang University;Urumchi 830046;China
..............page:1166-1172
Preparation and properties of novel human-like collagen-silk fibroin scaffold for blood vessel
Chenhui Zhu1;2;Daidi Fan1;2;Xiaoxuan Ma1;2;Wenjiao Xue2;Junfeng Hui1;2;Lan Chen3;Zhiguang Duan1;and Pu Ma4 1 Shaanxi Key Laboratory of Degradable Biomedical Materials;Northwest University;Xi’an 710069;China 2 Shaanxi Research & Development Center of Biomaterials and Fermentation Engineering;Northwest University;Xi’an 710069;China 3 College of Life Science;Northwest University;Xi’an 710069;China 4 School of Chemical Engineering;Northwest University;Xi’an 710069;China
..............page:1225-1233