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cha shi za jiao ji shu
Wang Zhi A potential useful and convenient differential hybridization technique for screening specific gene during normal development;cell differentiation and carcinogenesis has been discussed.Total 32P labeled cDNA’ of hepatoma 7777 was prepared and used as hybridization probe I. The enriched hepatoma cDNA was prepared from total cDNA of hepatoma 7777 by hybridizing it. with excess normal mRNA at Rot 1000 for 3 hrs at 68℃. Single stranded cDNA and hybrids were then separated on a hydroxyapatite column. The single stranded enriched hepatoma cDNA was used as hybridization probeⅡ.Both the probes to use have been discussed.
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