..............page:98-102
..............page:102-104
da dou zuo yuan dui yi bing shen shang xian su suo zhi xin ji fei hou da shu xue guan huo xing wu zhi de zuo yong
Huang Zhihua1;2;Li Xiao2;Zhong Xinming2;Zeng Jing2;Han Liming1;3 Object: To observe the protection of daidzein on rat’s myocardial hypertrophy caused by isopropylarterenol and explore the relationship of vasoactive substance.Method: The rat’s myocardial hypertrophy model is formed through hypodermic injection which extended 10 days at back skin with Iso;1 mg/kg.Once the model is formed;it will be administrated with various doses of DD;DMSO or NS through hypodermic injection from the second day;and the process will extend 14 days.After the last administration;the rat will be in absolute diet status for 12 h and its blood and heart are taken under anesthesia.The indexes of heart weight was calculated;the concentration of hydroxyproline in myocardium tissue was detected by alkali method;the level of angiotensin Ⅱ;endothelin;atrial natriuretic peptide and 6-keto-prostaglandin F1α;in plasma were investigated with radioimmunity kits;and the content of NO in serum was estimated by nitrate reductase assay.Result: There is obvious difference compare Iso group with control group.Indexes of heart weight and content of hydroxyproline of Iso group are much bigger than control one;level of ANP and NO are decreased while AngⅡ and ET are decreased.However;indexes of heart weight is decreased in DD groups;furthermore;ANP;NO and 6-keto-PGF1α level are enhanced while AngⅡ and ET concentration are reduced in DD groups.Conclusion: DD has a protection effect on rat’s myocardial hypertrophy caused by Iso;and its mechanism probably result of modulating the concentration of vasoactive substance such as AngⅡ;ANP;ET;NO and 6-keto-PGF1α.
..............page:37-39
..............page:111-115
..............page:108-111
..............page:115-118
..............page:104-106
..............page:118-121
..............page:121-124
..............page:106-108