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zuo xiang bao xin wan dui tang niao bing da shu xin ji xian wei hua de ying xiang
Liu Xiaoyan1;Wang Yuanzheng2;Han Yu1 Objective: To investigate the effects of ShexiangbaoXinwan on myocardial fibrosis of diabetic rats.Methods : Forty SD rats were randomly divided into the normal control group;the diabetic group;the diabetic group treated with low dose shexiangbaoxinwan and the diabetic group treated with high dose shexiangbaoxinwan;Rats in the last two groups were fed with shexiangbaoxinwan for 12 weeks.After the rats were killed;body weight;heart weight and left ventricular mass were weighted.The ratio of heart weight to body weight and left ventricular mass index were calculated.The myocardial interstitial collagen volume fraction were Measured with Masson methods.The ultrastructure of myocardial cell was observed with electron micscope.The concentration of AngiotensinII of regional myocardium were determined by radioimmunoassay.The expressions of TGF-β1 were determined by immunohistochemistry.Results Compared with the NC group;H/B;LVMI and myocardial interstitial CVF increased in the DM group.rats in the DM group had abnormal cardiac ultrastructure with confusion of my-ofibril and swelling and gathering of mitochondria.Expression of TGF-β1 and the concentration of AngII obviously increased.The above trends were significantly improved in the last two groups;and have a dose-dependent;High-dose group changes more obvious.Conclusion : Shexiangbaoxinwan Pill can inhibit diabetic myocardial fibrosis in rats;the mechanisms may relate with reducing the expression of TGF-β1 and AngⅡ.
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