China Animal Husbandry & Veterinary Medicine 2014 Issue 3 Development of SYBR GreenⅠ-based RT-PCR Assay for Detection of TIGAR mRNA in Swine

Development of SYBR GreenⅠ-based RT-PCR Assay for Detection of TIGAR mRNA in Swine
KANG Da-cheng;TANG Xiao-yan;LI Peng-ying;YAN Cheng-ying;FAN Cheng-ming;Institute of Quality Standards & Testing Technology for Agri-Products;Chinese Academy of Agricultural Sciences;Key Laboratory of Meat Processing and Quality Control;Ministry of Education;Nanjing Agricultural University;Institute of Crop Science;Chinese Academy of Agricultural Sciences;

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zhu fan zhi yu hu xi zong he zheng bing du gan ran de ju shi xi bao zhong microrna zu de te xing yan jiu
hicks a j; wu yan ;

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Developmental Expression Patterns of VLDLRand ApoER2 Genes in Duck Muscles
GOU Hua;HU Shen-qiang;PAN Zhi-xiong;LIU He-he;LI Liang;WANG Ji-wen;Key Laboratory of Animal Genetic Resources Exploitation and Utilization;Sichuan Agricultural University;

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ri liang tian jia su an suan dui zhu fan zhi yu hu xi zong he zheng bing du huo jun yi miao you dao de mian yi ying ji de ying xiang
mao x; sun peng ;

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Genetic Variation Analysis of S1 Genes of Porcine Epidemic Diarrhea Virus Isolated from Henan in 2012
CHEN Hui-juan;WU Zhi-ming;YAN Ruo-qian;ZHAO Xue-li;ZHAO Ming-jun;ZHOU Bing-qiang;KONG Gang-rui;YAN Zhi-ling;CHENG Jun-zhen;JIN Li-fen;College of Husbandry and Veterinary;Henan Agricultural University;Henan Center for Animal Disease Control and Prevention;

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Establishment of a Double Antibody Sandwich ELISA for the Detection of Flavobacterium columnaris
LV Na;YIN Xiao-ping;ZHANG Hong-xi;ZHANG Mao-lan;SUN Qiang;ZHAO Guo-kun;ZHANG Jie;College of Biological and Environmental Sciences;Zhejiang Wanli University;College of Food Science and Engineering;Jilin Agricultural University;

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zhu fan zhi yu hu xi zong he zheng bing du zai xin xian zhu rou zhong cun huo qing kuang de yan jiu
guarino h; wu yan ;

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guo yang hua yu mi tuo shui jiu jing zao ji qi ke rong wu he wei sheng su e shui ping dui duan nai zi zhu sheng chang xing neng zuo ti pin zhi ji rou zhi de ying xiang
song r; wu jiang li ;

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Preparation and Characteristics Identification of Anti-kappa Casein Monoclonal Antibody
ZHANG Ying;ZHOU Yu;LI Yan-song;MENG Xing-yu;CHU Xiao-hong;LU Shi-ying;REN Hong-lin;HU Pan;LIU Zeng-shan;WANG Nan-nan;LI Dan;Key Laboratory of Zoonosis;Ministry of Education;Institute of Zoonosis;College of Veterinary Medicine;Jilin University;

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Specific Detection of Shigella Using DPO-PCR Method
XU Yi-gang;LI Dan-dan;LIU Zhong-mei;WU Yan;LI Su-long;WEI Dong-xu;Technical Center of Heilongjiang Entry-exit Inspection and Quarantine Bureau;Technical Center of Hainan Entry-exit Inspection and Quarantine Bureau;

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ri liang dan bai zhi he lai an suan shui ping dui yan ge zi zhu sheng chang xing neng zuo ti pin zhi ji nei zhi fang he zhi fang suan zu cheng de ying xiang
tous n; wu jiang li ;

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Isolation,Identification and Genetic Variation Analysis of Two Strains of Porcine Reproductive and Respiratory Syndrome Virus
SUN Long;LIU Yu;HUANG Zhen;XIE Jie-xiong;CUI Jin;WEI Chun-ya;CHEN Yao;DENG Sheng-chao;SU Shuo;ZHANG Gui-hong;College of Veterinary Medicine;South China Agricultural University;Zhengzhou Sanquan Food Co.;Ltd.;

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Construction of Eukaryotic Expression Vector of Inner Mongolia Cashmere Goats KAP 1.1 Gene and its Gene Transfected
BAO A-dong;SU Zhi-fang;MEI Bu-jun;WANG Chang-shou;Department of Agronomy;Hetao College;

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Cloning and Expression Analysis of Buffalo Aquaporin 8 Gene
LI Sheng;QU Chun-feng;LI Run;HE Jin-na;SHI De-shun;LI Xiang-ping;State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources;Guangxi University;

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Clone of Tams1 Gene Non-hydrophobic Region of Theileria annulata and High Level Expression of Recombinant Protein GST-P27
CAO Wen-li;WANG Zhen;Shataer Kahaer;WANG Bing-jie;Bayinchahan;College of Veterinary Medicine;Xinjiang Agricultural University;Animal Husbandry and Veterinary Station of Turpan City;

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si wei yu mi jie gan he hun he cu si liao ri liang dui he si tan nai niu ru zhi fang suan zu cheng de ying xiang
han r w; sun peng ;

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Preliminary Study on Incompatibility Plasmids of 101 Strains of Salmonella typhimuriumby PCR-based Replicon Typing
LI Jing-yi;CUI Ke-qi;FENG Sai-xiang;HE Xian-hui;LIAO Ming;XU Cheng-gang;Key Laboratory of Veterinary Vaccine Innovation;Ministry of Agriculture;College of Veterinary Medicine;South China Agricultural University;

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Construction and Identification of Recombinant Adenovirus Expression Vector Carrying the Fusion Genes ORF2 and T Lymphocyte Epitopes of Porcine Circovirus Type 2
MO Yong-zheng;LUO Man-lin;CHEN Rui-ai;College of Veterinary Medicine;South China Agricultural University;Guangdong Dahuanong Animal Health Products Co.;Ltd.;

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Development of the Duplex Real-time RT-PCR Assay with TaqMan Probe of N-ITR Genes for Detection of Peste des Petits Ruminants Virus and Goat Pox Virus
ZHAO Wen-hua;YANG Shi-biao;GAO Hua-feng;WANG Jin-ping;Yunnan Tropical and Subtropical Animal Viral Disease Laboratory;Yunnan Academy of Animal Husbandry and Veterinary Medicine;

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Codon Optimization and Expression in BCG of E₀ Truncated Gene from C₂₄V Strain of Bovine Viral Diarrhea-Mucosal Disease Virus
ZHANG Yun;HAO Jun-wei;LIU Hong-na;WANG Wen-yu;YANG Yu-hang;SHI Kun;LI Jian-ming;DU Rui;College of Animal Science and Technology;Jilin Agricultural University;College of Chinese Medicinal Material;Jilin Agricultural University;

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Development of Duplex Real-time RT-PCR Assay for Detection of Duck Tembusu Virus and Duck Plague Virus
ZHANG Yan-fang;XIE Zhi-xun;XIE Li-ji;LIU Jia-bo;FAN Qing;PANG Yao-shan;LUO Si-si;DENG Xian-wen;XIE Zhi-qin;Guangxi Key Laboratory of Animal Vaccines and Diagnostics;Guangxi Veterinary Research Institute;

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Essential Amino Acid Upregulates L-type Amino Acid Transporter 1 Expression in Mammary Acinus
LIN Ye;HOU Xiao-ming;TIAN Lei;QI Bang-ruo;LI Qing-zhang;Key Laboratory of Dairy Science of Ministry of Education;Northeast Agricultural University;

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Cloning and Sequence Analysis of S1 Gene of Avian Nephropathogenic Infectious Bronchitis Virus QD Isolate
SUN Mei-yu;CHEN Bing;XING Jing-min;WANG Shou-shan;LV Mao-jie;HE Zhao-qing;YANG Bao-shou;Biological Products Research Center;Ringpu Biological Research Institute;Tianjin Ringpu Bio-technology Co.;Ltd.;

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Establishment of Loop-mediated Isothermal Amplification Assay for Detection of Salmonella
LI Jia-tong;WANG Nan;JIN Zhen-ai;XU Yao;SHAO Hong-ze;JIN Xin;College of Agriculture;Yanbian University;Jilin Province Center for Animal Disease Prevention and Control;Animal Health Supervision Institute of Hunchun City;Jilin Institute of Animal Husbandry and Veterinary Science Research;

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Isolation and Identification of Haemophilus parasuis, Study on its Inactivated Bacterin
LI Xiao-hua;LIN Biao-sheng;LIU Jian-kui;DAI Ai-ling;YANG Xiao-yan;College of Life Sciences;Longyan University;Fujian Key Laboratory of Preventive Veterinary Medicine and Biotechnology;

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mi ru jie duan diao kong niu ru zhi fang qiu zhi zhi zu cheng yu da xiao de xiang guan xing yan jiu
stahy r m; wu jiang li ;

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Development and Application of Duplex PCR for Canine Distemper Virus and Canine Coronavirus Detection
SUN Yuan-yuan;ZHAO Peng;LIU Jun;WANG Yun;QIAN Ke;Zhenjiang Entry-exit Inspection and Quarantine Bureau;

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Expression,Purification and Identification of Nucleocapsid Protein and Glycoprotein of Porcine Reproductive and Respiratory Syndrome Virus
ZHANG Hui;CUI Huan-zhong;LAN Hai-nan;YANG Yan-hong;FU Zhi-ling;ZHENG Xin;College of Animal Science and Technology;Jilin Agricultural University;

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Research Progress on Expression Mechanism of miRNA Related with Skeletal Muscle Development
ZHANG Yan-jun;HAN Zhi-ling;ZHANG Wen-guang;LI Jin-quan;College of Animal Science;Inner Mongolia Agricultural University;

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Construction of Small-tailed Han Sheep Leptin Receptor Gene Standard Plasmid and Standard Curve Using Real-time Quantitative PCR
ZHANG Gui-shan;XU Jing;LOU Yu-jie;ZHANG Shan-peng;JIANG Huai-zhi;College of Animal Science and Technology;Jilin Agricultural University;Changchun University of Science and Technology;

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niu xue qing bai dan bai he la gen guo yang hua wu mei tuo dian jie he wu dui jia zhuang xian ji su mian yi ce ding de ying xiang
kumari g l; xie bing ;

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Metagenomics and its Application Prospect
Siriguleng Yu;Temulun;MING Liang;YI Li;Jirimutu;Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease;Ministry of Agriculture;College of Veterinary Science;Inner Mongolia Agricultural University;Key Laboratory of Dairy Biotechnology and Engineering;Ministry of Education;Inner Mongolia Agricultural University;Inner Mongolia Camel Protection Association;

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Isolation and Biological Characteristics of a Phage against Salmonella enteritidis from Sawage Water
ZOU Shang-shu;XU Jia-yuan;WENG Ya-biao;LIN Rui-qing;College of Veterinary Medicine;South China Agricultural University;

..............page:119-123

elisa he ji yu lei bing du ke li de zhong he kang ti shi yan zai jian ce zi ran gan ran huo yi miao mian yi ren ru tou liu bing du chan sheng kang ti fang mian de xiang guan xing shi yan
zhao h; liu meng ;

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Construction and Characterization of the Yeast Two-hybrid cDNA Library of Vero E6 Cells
CHI Yan-bin;SHI Da;ZHU Qing-he;CHEN Jian-fei;SHI Hong-yan;ZHANG Xin;LI Chang-long;HAN Xiao;LIU Ning;ZHAO Xin;FENG Li;Division of Swine Infectious Diseases;State Key Laboratory of Veterinary Biotechnology;Harbin Veterinary Research Institute;Chinese Academy of Agricultural Sciences;College of Life Science;Northeast Agricultural University;

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Study on Favorable Protein Production Parameters from Apple Residue Fermented by Mixed Strains
XUE Zhu-lin;HUANG Bi-zhi;Department of Grass Science;Yunnan Agricultural University;Yunnan Academy of Grassland and Animal Science;

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Effects of Acidifier on Fresh-keeping of Feeds and Diarrhea in Growing Minks
WANG Kai-ying;BAO Kun;XU Chao;LIU Han-lu;YUE Zhi-gang;LI Guang-yu;State Key Laboratory of Specical Economic Animal Molecular Biology;Institute of Special Animal and Plant Science;CAAS;

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kang ti he mei biao ji wu de bu tong zu he dui yun tong mei lian mian yi xi fu shi yan de ying xiang
kumari g l; xie bing ;

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Research Progress on Immune Function of Vitamin A
ZHANG Yun-bo;LI Ming-wei;College of Agriculture;Guangdong Ocean University;

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Research Progress on Fat Nutrition in Monogastric Animal
ZHANG Ting;ZHONG Wei;ZHOU Ning;LI Guang-yu;Institute of Special Animal and Plant Sciences of CAAS;Agriculture College;Yanbian University;

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Research Progress on Yolk Antibody Feed Additives in China
WANG Jing;ZHAO Jian-guo;College of Agriculture;Hainan University;

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Study on Isolation,Identification and Enzyme-producing Conditions Optimized of Cellulolytic Bacteria in Ox Dung
GAO Yun-hang;ZHANG Xi-hong;WANG Wei;WANG Yan-qiu;MA Hong-xia;LOU Yu-jie;College of Animal Science and Technology;Jilin Agricultural University;Sub-district Office of Changchun Jingyue National High-technology Industrial Development Zone;

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Culture and Biological Characteristics Observation of Skin Fibroblast Cells from Tibetan Mastiff
SHI Fan;YANG Yong-mei;YU Hong-hao;GUO Zhi-lin;GUO Song-chang;ZHAO Xin-quan;Academy of Chemistry and Life Science;Qinghai University for Nationality;Key Laboratory of Adaptation and Evolution of Plateau Biota;Northwest Institute of Plateau Biology;Chinese Academy of Sciences;Academy of Public Management;Qinghai University for Nationality;Research Centre of Life Science;Yulin University;

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Effect of Glucose on Gene Expression and Enzyme Activity of GPX1 and SOD1 in Goose Primary Hepatocytes
WEN Rui;SHAN Shu-wen;HU Shen-qiang;LIU Dan-dan;LI Liang;HAN Chun-chun;WANG Ji-wen;Institute of Animal Genetics and Beeding;Sichuan Agricultural University;

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Study on the Effect of Hedyotis diffusa Willd Superfines Powder on Immune Function in Mice
WANG Hang;TANG Cheng;YUE Hua;WANG Hong-ge;School of Life Science and Technology;Southwest University for Nationalities;

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Effects of Daidzin and Genistin on Proliferation of Goat Mammary Gland Epithelial Cells
LI Yan;CHEN Feng;LV Xiao-feng;PENG Chun-yan;Supervision and Testing Center for Agricultural Product Quality;College of Animal Science and Technology;Yangzhou University;

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1zhong neng di kang zhi bing xing xin cheng yi bing du qie qu fen zi ran gan ran he yi miao mian yi de bing du yang ke li yi miao yan jiu
park j k; liu meng ;

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Research Progress on Reproductive Tract Associated Lymphoid Tissue in Chicken
YANG Shu-bao;ZHANG Ying-nan;MA Xin;LUAN Wei-min;QIAN Ai-dong;College of Animal Science and Technology;Jilin Agricultural University;Changchun University of Science and Technology;College of Life Science;Jilin Agricultural University;Key Laboratory of Animal Production & Product Quality and Security;Ministry of Education;Jilin Agricultural University;

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microrna-23 tong guo jian jie ba xiang prrsv rna huo ke neng tong guo xia diao xing gan rao su yi zhi prrsv fu zhi de yan jiu
zhang q; wu yan ;

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Research Progress of Pulmonary Surfactant Proteins
MENG Qing-xue;LIU Yu-fen;LIU Jing;WU Di;College of Life Science and Technology;Harbin Normal University;

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Establishment of Turpan Gamecock Aggression Behavior Spectrum and SNP Analysis of Monoamine Oxidase AGene
LI Yi-dan;LIU Xiu-xia;LI Ying-sheng;WANG Lei;JIA Wei;LI Xing-ming;ZHONG Xin-xian;LI Yan;LIAO He-rong;College of Life Sciences;Shihezi University;

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si wei dai ru liao dui du niu sheng chang xing neng liu wei fa yu he dai xie de ying xiang
silper b f; sun peng ;

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SNPs Detection of Thyroglobulin Gene and its Association with Growth Traits in Yanbian Yellow Cattles
XIA Guang-jun;JIN Xin;TIAN Wan-nian;JIN Hai-guo;YAN Chang-guo;Agricultural College;Yanbian University;College of Animal Science;Jilin Agricultural Science and Technology College;Branch of Animal Husbandry;Jilin Academy of Agricultural Science;

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zhu fan zhi yu hu xi zong he zheng bing du jie gou dan bai gp2 zai yi zhi diao wang zhong qian zai zuo yong de yan jiu
pujhari s; wu yan ;

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Cloning,Single Nucleotide Polymorphism and Bioinformatics Analysis of HSP70 Gene in Chicken
CHAO Zhe;LIN Zhe-min;LIU Hai-long;ZHANG Xing;ZHENG Xin-li;HUANG Li-li;XIE Liang;WANG Feng;Institute of Animal Science and Veterinary Medicine;Hainan Academy of Agricultural Sciences;Hainan Key Laboratory of Tropical Animal Reproduction &Breeding and Epidemic Disease Research;

..............page:194-199

Effects of Quinestrol on the Testes Development and Expression of PCNA and Caspase-3 in Juvenile Mice
ZHANG Fei-hu;LI Jian;JIN Guan-tong;WANG Hong-wei;ZHOU Bian-hua;LIU Yan-yan;HUANG Chun-yang;ZHAO Wen-peng;College of Animal Science and Technology;Henan University of Science and Technology;State Key Laboratory of Brain and Cognitive Science;Institute of Psychology;Chinese Academy of Sciences;

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The Molecular Mechanisms Underlying Methylation Intervention in the Cell Differentiation
JI Bin;SUN Ting-ting;CHEN Pei-fu;College of Animal Science and Technology;Yunnan Agricultural University;

..............page:203-207

Research Development and Application of the Deep Insemination Technology in Pig
ZHANG Ying-ying;Zhengzhou City Animal Husbandry Technology Centre;

..............page:207-212

Research Progress on Biological Characteristics of Mink Calicivirus
ZHUANG Jin-qiu;MEI Jian-guo;SHEN Zhi-qiang;Shandong Binzhou Animal Science & Veterinary Medicine Academy;

..............page:213-216

Study on Moderate Exercise to Reduce Influenza Animal Lung Inflammatory Injury in Mice Model
HU Guang-xia;GU Ke-peng;Jilin University;Jilin Agricultural University;

..............page:217-219

Research Progress on Virulence Factors of Acinetobacter baumannii
ZOU De-ying;LIU Dong;GUO Xing;CHEN Xiao-feng;HU Pan;LU Shi-ying;ZHOU Yu;LIU Zeng-shan;REN Hong-lin;Key Laboratory of Zoonosis Research;Ministry of Education;Institute of Zoonosis;College of Veterinary Medicine;Jilin University;

..............page:220-222

Research Progress and Vaccine Prospect of Porcine Epidemic Diarrhea Virus
CHEN Shen-miao;NIU Cheng-ming;HE Fu-qing;WU Xue-li;MA Jing-xia;Shandong Binzhou Wohua Biotech Co.;Ltd.;Shandong Binzhou Bilaiwei Biological Technology Institute;Animal Husbandry and Veterinary Bureau;

..............page:223-229

Effects of Different Doses of Chinese Herbal Medicine on Immune Efficiency of Newcastle Disease Oil Emulsion Inactivated Vaccine in Laying Hens
JIA Wan-chen;SHI Zhao-guo;LIU Gen-xin;College of Animal Science and Technology;Gansu Agricultural University;Gansu Polytechnic College of Animal Husbandry and Engineering;

..............page:230-232

Antibacterial Study of Different BidensbipinnataL Extracts against Drug-resistant E.coli
ZHENG Yan-qing;ZOU Zhi-ming;HE Jia-kang;SONG Jian-wu;SI Hong-bin;College of Animal Science and Technology;Guangxi University;

..............page:233-236

yi zhong ji yu xin xing ban kang yuan he dan ke long kang ti de yong yu shi yong dong wu zu zhi zhong huang an lei yao wu jian ce de elisa fang fa de jian li
zhou q; xie bing ;

..............page:236

Reaserch Progress on Cefovecin
SONG Fu-jie;LIU Ming-chun;FU Te;Luoding Polytechnic College;College of Animal Husbandry and Veterinary;Shenyang Agricultural University;

..............page:237-241

Research Progress on Chemical Components and Biological Activity of Rhodomyrtus tomentosa
LIU Wei;ZHAO Wu;MENG Fei;YIN Hui-hui;JIANG Yuan-ming;SUN Jian-hua;Guangxi Key Laboratory of Animal Vaccine and New Technology;Guangxi Veterinary Research Institute;

..............page:241-244

Comparative Tests of Vaccines Prepared with Domestic and Import Oil Adjuvants
YIN Xiu-feng;DING Mei-juan;HUANG Xian-ming;ZHANG Xiao-fei;XUE Jia-bin;XU Wen-long;ZHANG Xiao-yi;Nanjing Tianbang Bio-industry Co.;Ltd.;

..............page:245-247

Serological Survey on Three Viral Diarrhea Diseases of Yaks in Northwest Sichuan Province
HE Mei-lin;ZHANG Huan-rong;WANG Yong;WANG Yan-xuan;WANG Yuan-wei;TANG Cheng;College of Life Science and Technology;Southwest University for Nationalities;Veterinary Medicine Key Laboratory of Sichuan Institutes of Higher Learning;

..............page:248-251

Development and Production of Sunflower By-product Feed Resource in Hetao Area in Inner Mongolia
MA Hui-ru;ZHAO Zhi-xiang;CHEN Yan-jun;Department of Agronomy;Hetao College;

..............page:251-254

Study on the Extraction Process of Compound Epimedium by Orthogonal Experimental Design
GU Yu-feng;HUANG Ya-jun;WANG Qin;WU Yong;LIU Zhao-ying;ZHANG Ming-jun;SUN Zhi-liang;JIANG Zheng-yun;Veterinary Facultry;Hunan Agricultural University;Hunan Engineering Research Center of Veterinary Drug;Animal Health Engineering and Technology Research Center;Hunan Agricultural University;

..............page:255-258

Current Research Status of Brucella Vaccine
FU Ying-ying;QIN Gui-xin;QIAN Jun;ZHAO Si-yan;ZHENG Guan-yu;SUN Yu-cheng;WAN Jia-yu;LIU Wen-sen;LIU Lin-na;College of Animal Science and Technology;Jilin Agricultural University;Institute of Military Veterinary;Academy of Military Medical Science;Key Laboratory of Jilin Province for Zoonosis Prevention and Control;

..............page:258-261

Application of Urea in Dairy Cows
YANG De-zhi;ZHOU Ling-yun;LI Fa-di;State Key Laboratory of Animal Nutrition;Institute of Animal Sciences;Chinese Academy of Agricultural Sciences;College of Animal Science and Technology;Gansu Agricultural University;Livestock Technology Extention Center of Qingyang City;College of Pastoral Agriculture Science and Technology;Lanzhou University;

..............page:262-266

ao te qi zhuan zhu mei jun du su zhuan ye cheng jiu wei lai cai fang ao te qi mei jun du su guan li tuan dui quan qiu xiao shou zong jian nick adams xian sheng
zhao lian sheng ; wen jing ;

..............page:267-268