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Chinese Journal of Biotechnology
1000-3061
2010 Issue 2
Construction and expression analysis of the zebrafish heart-specific transgenetic vector based on Tol2 transposable element
Tingfang Chen; Na Luo; Huaping Xie; Xiushan Wu; and Yun Deng College of Life Sciences; Hunan Normal University; Changsha 410081; China
..............page:230-236
Enhanced production of taxuyunnanine c in cell suspension cultures of Taxus chinensis by methyl jasmonate elicitation and in situ absorption
Mingbo Gao1;2;3; Wei Zhang1;4; and Xingju Yu1 1 Marine Bioproducts Engineering Group; Dalian Institute of Chemical Physics; Chinese Academy of Sciences; Dalian 116023; China 2 Graduate School of the Chinese Academy of Sciences; Beijing 100049; China 3 College of Life Science; Dalian Nationalities University; Dalian 116600; China 4 Molecular Bioprocessing and Bioproducts Laboratory; Department of Medical Biotechnology; School of Medicine; Flinders University; Adelaide; SA 5042; Australia
..............page:223-229
Engineering and metabolic characteristics of a Clostridium tyrobutyricum strain
Guiqing Yang1;2; Gang Liu1; and Changde Yang1 1 Shenzhen Key Laboratory of Microbial Genetic Engineering; College of Life Sciences; Shenzhen University; Shenzhen 518060; China 2 Shenzhen Luohu Center For Disease Control Prevention; Shenzhen 518020; China
..............page:170-176
Stable expression and characterization of the von Willebrand factor cleaving protease
Zhenni Ma; Ningzheng Dong; Jingyu Zhang; Jian Su; Anyou Wang; and Changgeng Ruan Key Laboratory of Thrombosis and Hemostasis; Ministry of Health; Jiangsu Institute of Hematology; the First Affiliated Hospital of Soochow University; Suzhou 215006; China
..............page:244-248
Localization of polypeptides release factors and ribosome protein L11 in Euplotes octocarinatus
Baofeng Chai; Na Li; Jingtao Wang; Quan Shen; Zhiyun Zhang; and Aihua Liang Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education; Institute of Biotechnology; Shanxi University; Taiyuan 030006; China
..............page:237-243
Kinetic mechanisms of glycerol dehydrogenase and 1,3-propanediol oxidoreductase from Klebsiella pneumoniae
Hongwen Chen; Jinfeng Nie; Guo Chen; and Baishan Fang Key Laboratory of Industrial Biotechnology; Fujian Province; Huaqiao University; Xiamen 361021; China
..............page:177-182
Screening of collagenase-producing strain and purification of Bacillus cereus collagenase
Lili Liu1;2; Meihu Ma1; Xiufang Yu1; and Wentao Wang1 1 College of Food Science & Technology; Huazhong Agricultural University; Wuhan 430070; China 2 School of Food & Bioengineering; Henan University of Science and Technology; Luoyang 471003; China
..............page:194-200
Expression, purification and application of blaTEM-116 extended-spectrum β-lactamase
Zhen Wang1; Ying Zheng2; Quanshui Fan2; Xiushu Chen1; and Jianxin Lu1 1 Institute of Microecology; School of Laboratory Medicine and Life Science; Wenzhou Medical College; Wenzhou 325035; China 2 The Medical Institute of Chengdu Military Area; Kunming 650032; China
..............page:256-263
Expression, purification and renaturation of Pol P51 antigen of HIV-1 strain CN54 and its application in antibody detection
Jue Hou1;2; Jing Sun2; Zhiyong Xu2; Wenling Fan2; Yixuan Zhang1; Yong Liu2; and Yanling Hao2 1 College of Life Science and Biopharmaceutics; Shenyang Pharmaceutical University; Shenyang 110016; China 2 State Key Laboratory for Infectious Disease Prevention and Control; National Center for AIDS/STD Control and Prevention; Chinese Center for Disease Control and Prevention; Beijing 100050; China
..............page:201-206
Biomass carbohydrates assimilation and lipid accumulation by Mortierella isabellina
Dahui Xing1; Anlong Pan1; Donghua Xue1; Mingyu Fang1; and Ranfeng Gu2 1 School of Chemistry and Life Science; Changchun University of Technology; Changchun 130012; China 2 Changchun Changsheng Gene Pharmaceutical Company Limited; Changchun 130012; China
..............page:189-193
Surface display of GFP using CotX as a molecular vector on Bacillus subtilis spores
Qian Li1;2; Degang Ning1; and Chundu Wu1 1 School of Environment; Jiangsu University; Zhenjiang 212013; China 2 Institute of Life Science; Jiangsu University; Zhenjiang 212013; China
..............page:264-269
Separation of magnetic bacteria by using a magnetic separator
Xinxing Liu; Ning Guo; Yingjie Yang; Wanjie Liang; and Jian Zhang School of Minerals Processing and Bioengineering; Central South University; Changsha 410083; China
..............page:270-275
Regulatory networks of somatic embryogenesis in plant
Xingchun Wang; Hong Li; Min Wang; and Zhirong Yang 1 College of Life Sciences; Shanxi Agricultural University; Taigu 030801; China 2 College of Arts & Sciences; Shanxi Agricultural University; Taigu 030801; China
..............page:141-146
Improving ethanol tolerance of Saccharomyces cerevisiae industrial strain by directed evolution of SPT3
Xinqing Zhao; Rujiao Jiang; Ning Li; Qing Yang; and Fengwu Bai Department of Bioscience and Bioengineering; Dalian University of Technology; Dalian 116024; China
..............page:159-164
High level expression of recombinant human kallistatin in Pichia pastoris and its bioactivity
Xiaoping Huang1; Xiao Wang1; Hao Dong1; Xiaofeng Zhao1; Zhaofa Li1; 2; Qizhao Wang1;2; Ruian Xu1;2; and Yong Diao1;2 1 Institute of Molecular Medicine; Huaqiao University; Quanzhou 362021; China 2 Molecular Medicine Engineering Research Center of the Ministry of Education; Huaqiao University; Quanzhou 362021; China
..............page:249-255
Eukaryotic expression and bioactivity determination of the fusion protein sTNFRII-gAD consisting of soluble tumor necrosis factor receptor II and globular domain of adiponectin
Suyun Chen1;2; Qiushan He3; Xiaoyan Dong2;4; Xiaobing Wu2; and Jimin Gao1;3 1 Zhejiang Provincial Key Laboratory of Medical Genetics; Wenzhou Medical College; Wenzhou 325035; China2 National Laboratory of Molecular Virology and Genetic Engineering; Institute for Viral Disease Control and Prevention; Chinese Center for Disease Control and Prevention; Beijing 100052; China 3 School of Biotechnology; Southern Medical University; Guangzhou 510515; China 4 Beijing Fiveplus Molecular Medicine Institute; Beijing 100176; China
..............page:207-215
Effect of cadmium, alone or in combination with CaCl2, on the growth, antioxidative enzyme activity and cadmium absorption of Solanum nigrum L. var pauciflorum hairy roots
Heping Shi1;2; Eric Pokeung Tsang2; Yunling Wang1; and Andrew Leewah Chan2 1 Guangdong Provincial Key Laboratory of Biotechnology for Plant Development; College of Life Science; South China Normal University; Guangzhou 510631; China 2 Department of Science and Environmental Studies; the Hong Kong Institute of Education; New Territories; Hong Kong; China
..............page:147-158
Cloning, expression and evaluation of Saccharomyces cerevisiae ADH2
Mengbin Yu; Qingwen Zhi; Li Xu; Chuangxin Zhao; Gaoyun Chen; Yongchao Jiang; and Min Liu Laboratory of Biology Defense; Command and Engineering College of Chemical Defense; Beijing 102205; China
..............page:165-169
Development of a fed-batch process for TNFR-Fc producing GS-CHO cells
Li Fan; Liang Zhao; Yating Sun; Tianci Kou; and Wensong Tan State Key Laboratory of Bioreactor Engineering; East China University of Science and Technology; Shanghai 200237; China
..............page:216-222
Breeding of ammonium-tolerant mutants of Actinobacillus succinogenes for succinic acid production and effect of ammonium
Guizi Ye; Min Jiang; Kequan Chen; Jian Li; Yonglan Xi; Xiumei Huang; and Ping Wei State Key Laboratory of Materials-oriented Chemical Engineering; College of Life Science and Pharmaceutical Engineering; Nanjing University of Technology; Nanjing 210009; China
..............page:183-188