1000-3061
2009 Issue 7
Strategy and application of metaproteomics
Rentao Yu1; Peiji Gao2; Li Han1 and Liuyu Huang1 1 Center for Hospital Infection Control; the Institute for Disease Prevention and Control of PLA; Beijing 100071; China 2 The State Key Laboratory of Microbial Technology; Shandong University; Jinan 250100; China
Rentao Yu1; Peiji Gao2; Li Han1 and Liuyu Huang1 1 Center for Hospital Infection Control; the Institute for Disease Prevention and Control of PLA; Beijing 100071; China 2 The State Key Laboratory of Microbial Technology; Shandong University; Jinan 250100; China
..............page:961-967
Expression of acetohydroxyacid synthase isozyme genes ilvBN,ilvGM,ilvIH and their resistance to AHAS-inhibitor herbicides
Jingjing Shen; Yongfeng Li; Xing Huang; Xinyan Yu; Jian He; and Shunpeng Li Key Laboratory of Microbiological Engineering of Agricultural Environment; Ministry of Agriculture; Microbiology Department; College of Life Science; Nanjing Agricultural University; Nanjing 210095; China
Jingjing Shen; Yongfeng Li; Xing Huang; Xinyan Yu; Jian He; and Shunpeng Li Key Laboratory of Microbiological Engineering of Agricultural Environment; Ministry of Agriculture; Microbiology Department; College of Life Science; Nanjing Agricultural University; Nanjing 210095; China
..............page:1007-1013
C3d-M28 enhanced DNA vaccination induced humoral immune response to glycoprotein C of pseudorabies virus
Huiying Fan1*; Zhongyong Liu2*; Tiezhu Tong3;4; Xing Liu3; and Aizhen Guo4 1 College of Veterinary Medicine; South China Agricultural University; Guangzhou 528427; China 2 Guangdong Entre-exit Inspection and Quarantine Bureau; Guangzhou 541004; China 3 Huizhou Entre-exit Inspection and Quarantine Bureau; Huizhou 516006; China 4 College of Veterinary Medicine; Huazhong Agricultural University; Wuhan 430070; China
Huiying Fan1*; Zhongyong Liu2*; Tiezhu Tong3;4; Xing Liu3; and Aizhen Guo4 1 College of Veterinary Medicine; South China Agricultural University; Guangzhou 528427; China 2 Guangdong Entre-exit Inspection and Quarantine Bureau; Guangzhou 541004; China 3 Huizhou Entre-exit Inspection and Quarantine Bureau; Huizhou 516006; China 4 College of Veterinary Medicine; Huazhong Agricultural University; Wuhan 430070; China
..............page:987-992
Effect of biological pretreatment with Trametes vesicolor on the enzymatic hydrolysis of softwood and hardwood
Hongbo Yu; and Xiaoyu Zhang College of Life Science and Technology; Huazhong University of Science and Technology; Wuhan 430074; China
Hongbo Yu; and Xiaoyu Zhang College of Life Science and Technology; Huazhong University of Science and Technology; Wuhan 430074; China
..............page:993-998
Prokaryotic expression and polyclonal antibody preparation of TRαA in Japanese flounder Paralichthys olivaceus
Liang Jia; Zhiyi Shi; and Junling Zhang Biotechnology Research Center; Shanghai Ocean University; Shanghai 201306; China
Liang Jia; Zhiyi Shi; and Junling Zhang Biotechnology Research Center; Shanghai Ocean University; Shanghai 201306; China
..............page:999-1006
Cloning and expression of a gene encoding shortened LfcinB(1-15)-Melittin(5-12) hybrid peptide in Escherichia coli BL21(DE3)
Chongpeng Bi; Xingjun Feng; Anshan Shan; and Jiayin Guo Institute of Animal Nutrition; Northeast Agricultural University; Harbin 150030; China
Chongpeng Bi; Xingjun Feng; Anshan Shan; and Jiayin Guo Institute of Animal Nutrition; Northeast Agricultural University; Harbin 150030; China
..............page:975-981
Stable inhibition of human prion protein through a retrovirus-based RNAi system
Wenjing Xu1;2; Di Wang3; Juan Wang1;2; and Huaiyi Yang1 1 Institute of Microbiology; Chinese Academy of Sciences; Beijing 100101; China 2 Graduate University of Chinese Academy of Sciences; Beijing 100039; China 3 Beijing University of Chinese Medicine; Beijing 100029; China
Wenjing Xu1;2; Di Wang3; Juan Wang1;2; and Huaiyi Yang1 1 Institute of Microbiology; Chinese Academy of Sciences; Beijing 100101; China 2 Graduate University of Chinese Academy of Sciences; Beijing 100039; China 3 Beijing University of Chinese Medicine; Beijing 100029; China
..............page:1022-1027
Purification, crystallographic analysis of rhesus MHC-I Mamu-A~*02 complexed with simian immunodeficiency virus nonapeptide
Lianpan Dai1;2; Bin Zhou1; Jianxun Qi1; Ying Ma1; George F Gao1; and Xicai Yang1 1 Institute of Microbiology; Chinese Academy of Sciences; Beijing 100101; China 2 Graduate University of Chinese Academy of Sciences; Beijing 100039; China
Lianpan Dai1;2; Bin Zhou1; Jianxun Qi1; Ying Ma1; George F Gao1; and Xicai Yang1 1 Institute of Microbiology; Chinese Academy of Sciences; Beijing 100101; China 2 Graduate University of Chinese Academy of Sciences; Beijing 100039; China
..............page:1028-1034
Construction of adenovirus vector expressing IL-24 and E1A and its inhibition of SMMC-7721
Xiaohua Wang1; Jingcheng Miao2; Yufeng Xie2; Weihua Sheng2; Yunbo Shan2; and Jicheng Yang2 1 No.1 Affiliated Hospital of Soochow University; Suzhou 215001; China 2 Department of Celluar and Molecular Biology; Medical School; Suzhou University; Suzhou 215123; China
Xiaohua Wang1; Jingcheng Miao2; Yufeng Xie2; Weihua Sheng2; Yunbo Shan2; and Jicheng Yang2 1 No.1 Affiliated Hospital of Soochow University; Suzhou 215001; China 2 Department of Celluar and Molecular Biology; Medical School; Suzhou University; Suzhou 215123; China
..............page:1035-1041
..............page:1006
..............page:992
..............page:1106
Effect of salicylic acid on cell growth and polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense
Bo Wang; Lihua Pan; Jianping Luo; and Xueqiang Zha School of Biotechnology and Food Engineering; Hefei University of Technology; Hefei 230009; China
Bo Wang; Lihua Pan; Jianping Luo; and Xueqiang Zha School of Biotechnology and Food Engineering; Hefei University of Technology; Hefei 230009; China
..............page:1062-1068
Metabolic characteristics of GS-NS0 Myeloma cells producing anti-CD25 monoclonal antibody in serum-free culture
Liang Zhao; Li Fan; Xu Zhang; and Wensong Tan State Key Laboratory of Bioreactor Engineering; East China University of Science and Technology; Shanghai 200237; China
Liang Zhao; Li Fan; Xu Zhang; and Wensong Tan State Key Laboratory of Bioreactor Engineering; East China University of Science and Technology; Shanghai 200237; China
..............page:1069-1076
Preparation and detection of phosphorylated PRAS40 (Ser183) polyclonal antibody
Hao Wei; Bei Huang; Changzhi Xu; Zhuxia Zheng; and Yu Bai School of Life Science; Anhui University; Hefei 230039; China
Hao Wei; Bei Huang; Changzhi Xu; Zhuxia Zheng; and Yu Bai School of Life Science; Anhui University; Hefei 230039; China
..............page:1049-1054
Construction and expression of disulphide stabilized anti-CD3/anti-Pgp diabody
Ye Su; Juanni Liu; Yingdai Gao; Li Qin; Ming Yang; Jinhong Wang; Yuanfu Xu; Xiaofeng Shao; Qing Ji; Dongsheng Xiong; and Chunzheng Yang State Key Laboratory of Experimental Hematology; Institute of Hematology & Hospital of Blood Diseases; Chinese Academic of Medical Sciences & Peking Union Medical College; Tianjin 300020; China
Ye Su; Juanni Liu; Yingdai Gao; Li Qin; Ming Yang; Jinhong Wang; Yuanfu Xu; Xiaofeng Shao; Qing Ji; Dongsheng Xiong; and Chunzheng Yang State Key Laboratory of Experimental Hematology; Institute of Hematology & Hospital of Blood Diseases; Chinese Academic of Medical Sciences & Peking Union Medical College; Tianjin 300020; China
..............page:1042-1048
..............page:1094
Immobilization and characterization of carbonic anhydrase on the surface of hollow fiber membrane of polymethyl pentene
Qinmei Wang 1;2; Dihua Zhang 1; and Jingxia Zhang 3 1 First Affiliated Hospital; Sun Yat-sen University; Guangzhou 510080; China 2 Institute of Cardiology and Angiology; Sun Yat-sen University; Guangzhou 510080; China 3 School of Pharmaceutical Sciences; Sun Yat-sen University; Guangzhou 510080; China
Qinmei Wang 1;2; Dihua Zhang 1; and Jingxia Zhang 3 1 First Affiliated Hospital; Sun Yat-sen University; Guangzhou 510080; China 2 Institute of Cardiology and Angiology; Sun Yat-sen University; Guangzhou 510080; China 3 School of Pharmaceutical Sciences; Sun Yat-sen University; Guangzhou 510080; China
..............page:1055-1061
..............page:1013
..............page:1041,1054,1061,1068,1076
..............page:1113-1114
Microfluidic chip for detecting the expression of green fluorescent protein in Bacillus subtilis
Huijun Dong 1*; Jinglin Fu 2*; Yongquan Li 3; and Junyun Jiang 1 1 Research and Development Center; Lu’nan Pharmaceutical Group Inc. Ltd.; Linyi 276005; China 2 Institute of Microanalytical Systems; Zhejiang University; Hangzhou 310058; China 3 College of Life Sciences; Zhejiang University; Hangzhou 310058; China
Huijun Dong 1*; Jinglin Fu 2*; Yongquan Li 3; and Junyun Jiang 1 1 Research and Development Center; Lu’nan Pharmaceutical Group Inc. Ltd.; Linyi 276005; China 2 Institute of Microanalytical Systems; Zhejiang University; Hangzhou 310058; China 3 College of Life Sciences; Zhejiang University; Hangzhou 310058; China
..............page:1077-1081
Development and application of a mammlian one hybrid-based high-throughput screening model for Erα modulator
Qian Zhang1; Xiaoxi Shui1; Yuling Fan2; Weili Hao1; Zhihui Zheng1;2; Xinhua Lu2; Baohua Zhao1; Hua Zhang2; and Jiangong He2 1 College of Life Sciences; Hebei Normal University; Shijiazhuang 050016; China 2 Drug Research & Development Center of North China Pharmaceutical Group Corporation; National Microbial Medicine Engineering &Research Center; Shijiazhuang 050013; China
Qian Zhang1; Xiaoxi Shui1; Yuling Fan2; Weili Hao1; Zhihui Zheng1;2; Xinhua Lu2; Baohua Zhao1; Hua Zhang2; and Jiangong He2 1 College of Life Sciences; Hebei Normal University; Shijiazhuang 050016; China 2 Drug Research & Development Center of North China Pharmaceutical Group Corporation; National Microbial Medicine Engineering &Research Center; Shijiazhuang 050013; China
..............page:1088-1094
Optimization of porcine embryo vitrification
Defu Zhang1;2; Jianjun Dai1;2; Caifeng Wu1;2; Huali Wu1;2; Dong Liu1;2; Yu Yang3; Tingyu Zhang3; Wei Liu4; Fangzhi Yin4; Shaobing Wang4; and Shaokai Wang4 1 Animal Husbandry and Veterinary Research Institute; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China 2 Division of Animal Genetic Engineering; Shanghai Municipal Key Laboratory of Agri-genetic and Breeding; Shanghai 201106; China 3 Animal Sciences & Technology College; Sichuan Agricultural University; Ya’an 625014; China 4 Animal Sciences& Technology College; Nanjing Agricultural University; Nanjing 210095; China
Defu Zhang1;2; Jianjun Dai1;2; Caifeng Wu1;2; Huali Wu1;2; Dong Liu1;2; Yu Yang3; Tingyu Zhang3; Wei Liu4; Fangzhi Yin4; Shaobing Wang4; and Shaokai Wang4 1 Animal Husbandry and Veterinary Research Institute; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China 2 Division of Animal Genetic Engineering; Shanghai Municipal Key Laboratory of Agri-genetic and Breeding; Shanghai 201106; China 3 Animal Sciences & Technology College; Sichuan Agricultural University; Ya’an 625014; China 4 Animal Sciences& Technology College; Nanjing Agricultural University; Nanjing 210095; China
..............page:1095-1100
Genetic transformation of Nicotiana tabacum L.by Agrobacterium tumefaciens carrying genes in the melatonin biosynthesis pathway and the enhancement of antioxidative capability in transgenic plants
Yingjuan Wang1; Jingfen Jia1; Huaiyu Bu1; Yuwei Zhao1; Yao Xu2; Carl Hirschie Johnson2; and Jan Kolár3 1 Shaanxi Provincial Key Laboratory of Biotechnology; College of Life Science; Northwest University; Xi’an 710069; China 2 Department of Biology; Box 1812-B; Vanderbilt University; Nashville; TN 37235; USA 3 Institute of Experimental Botany; Academy of Sciences of the Czech Republic; Rozvojová 135; 165 02 Praha 6; Czech Republic
Yingjuan Wang1; Jingfen Jia1; Huaiyu Bu1; Yuwei Zhao1; Yao Xu2; Carl Hirschie Johnson2; and Jan Kolár3 1 Shaanxi Provincial Key Laboratory of Biotechnology; College of Life Science; Northwest University; Xi’an 710069; China 2 Department of Biology; Box 1812-B; Vanderbilt University; Nashville; TN 37235; USA 3 Institute of Experimental Botany; Academy of Sciences of the Czech Republic; Rozvojová 135; 165 02 Praha 6; Czech Republic
..............page:1014-1021
Ssp DnaB intein-mediated ligation of heavy and light chains of coagulation factor VIII in Escherichia coli
Fuxiang Zhu1; Zelong Liu1; Huige Qu1; Xiaolin Xin1; Hongxin Dong1; and Xiangqin Liu2 1 Life Science College of Ludong University; Yantai 264025; China 2 Department of Biochemistry and Molecular Biology; Dalhousie University; Halifax B3H 4H7; Canada
Fuxiang Zhu1; Zelong Liu1; Huige Qu1; Xiaolin Xin1; Hongxin Dong1; and Xiangqin Liu2 1 Life Science College of Ludong University; Yantai 264025; China 2 Department of Biochemistry and Molecular Biology; Dalhousie University; Halifax B3H 4H7; Canada
..............page:1101-1106
Expression, purification and immunogenicity analysis of HPV type 18 virus-like particles from Escherichia coli
Minghui Xie; Shaowei Li; Wentong Shen; Zhongyi Li; Yudi Zhuang; Xiaobing Mo; Ying Gu; Ting Wu; Jun Zhang; and Ningshao Xia National Institute of Diagnostics and Vaccine Development in Infectious Diseases; Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering; School of Life Science; Xiamen University; Xiamen 361005; China
Minghui Xie; Shaowei Li; Wentong Shen; Zhongyi Li; Yudi Zhuang; Xiaobing Mo; Ying Gu; Ting Wu; Jun Zhang; and Ningshao Xia National Institute of Diagnostics and Vaccine Development in Infectious Diseases; Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering; School of Life Science; Xiamen University; Xiamen 361005; China
..............page:1082-1087
Construction of an infectious cDNA clone derived from foot-and-mouth disease virus O/QYYS/s/06
Shousheng Lu1;2; Qizu Zhao3; Xiangtao Liu4; Yanwei Sun2; Tao Ren1; Guihong Zhang1; Wenbao Qi1; Yunfeng Zha2; Lingchen Kong2; Han Zhang2; Huiying Fan1; and Ming Liao1 1 College of Veterinary Medicine; South China Agricultural University; Guangzhou 510642; China 2 Guangdong Provincial Veterinary Station of Epidemic Prevention & Supervision; Guangzhou 510230; China 3 China Institute of Veterinary Drug Control; Beijing 100081; China 4 Lanzhou Veterinary Research Institute; Chinese Academy of Agricultural Sciences; Lanzhou 730046; China
Shousheng Lu1;2; Qizu Zhao3; Xiangtao Liu4; Yanwei Sun2; Tao Ren1; Guihong Zhang1; Wenbao Qi1; Yunfeng Zha2; Lingchen Kong2; Han Zhang2; Huiying Fan1; and Ming Liao1 1 College of Veterinary Medicine; South China Agricultural University; Guangzhou 510642; China 2 Guangdong Provincial Veterinary Station of Epidemic Prevention & Supervision; Guangzhou 510230; China 3 China Institute of Veterinary Drug Control; Beijing 100081; China 4 Lanzhou Veterinary Research Institute; Chinese Academy of Agricultural Sciences; Lanzhou 730046; China
..............page:982-986
Construction of HBD-3 gene mammary-specific expression vector and eukaryotic expression
Wei Peng; Zhigang Lan; Jingjing Ma; Baolei Wang; and Yong Zhang Institute of Biotechnology; College of Animal Veterinary Medicine; Northwest Agiculture and Forestry University; Yangling 712100; China
Wei Peng; Zhigang Lan; Jingjing Ma; Baolei Wang; and Yong Zhang Institute of Biotechnology; College of Animal Veterinary Medicine; Northwest Agiculture and Forestry University; Yangling 712100; China
..............page:968-974
..............page:967
Morphology and mechanical properties of normal lymphocyte and Jurkat revealed by atomic force microscopy
Xiaofang Cai; Jiye Cai; Shisong Dong; Hua Deng; and Mingqian Hu Department of Chemistry; Jinan University; Guangzhou 510632; China
Xiaofang Cai; Jiye Cai; Shisong Dong; Hua Deng; and Mingqian Hu Department of Chemistry; Jinan University; Guangzhou 510632; China
..............page:1107-1112
..............page:1087
..............page:1081