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Chinese Journal of Biotechnology
1000-3061
2009 Issue 10
Real time ex vivo detection and dynamic monitoring of in vivo expression of secreted luciferase gene injected by hydrodynamic method
Wenhong Tian1*; Gang Wang1*; Shuntao Luo1;2; Xiaoyan Dong1;3; Xinyang Fu1; Wenjie Tan1; and XiaobingWu1 1 Institute for Viral Disease Control and Prevention; Chinese Center for Disease Control and Prevention; National Laboratory of Molecular Virology and Genetic Engineering; Beijing 100052; China 2 State Key Laboratory of Biotherapy and Cancer Center; West China Hospital; West China Medical School; Sichuan University; Chengdu 610064; China 3 Beijing Fiveplus Molecular Medicine Institute; Beijing 100176; China
..............page:1552-1557
Chitosan and its applications in synthesis of metal nanomaterials
Dongwei Wei 1; Cuiying Jia 1; Xueping Jia 2; Yongzhong Ye 1; and Weiping Qian 3 1 College of Life Science; Henan Agricultural University; Zhengzhou 450002; China 2 School of Chemistry and Chemical Engineering; Nantong University; Nantong 226007; China 3 School of Biological Science and Medical Engineering; Southeast University; Nanjing 210096; China
..............page:1449-1458
Interleukin 24 inhibits growth and induces apoptosis of osteosarcoma cells MG-63 in vitro and in vivo
Yali Han; Jingcheng Miao; Weihua Sheng; Xiaohua Wang; Yingying Jing; Yunbo Shan; Tielian Liu; Wanrong Bao; and Jicheng Yang Cell and Molecular Biology Institute; College of Medicine; Soochow University; Suzhou 215123; China
..............page:1538-1545
Application of the self excision Cre/lox system in plants
Xiuming Liu1; Xinxin Meng1; Haiyan Li1;2; Jing Yang1; Hongqi Fu1; and Xiaokun Li1;3 1 Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development; Jilin Agricultural University; Changchun 130118; China 2 College of Life Sciences; Jilin Agricultural University; Changchun 130118; China 3 School of Pharmaceutical Sciences; Wenzhou Medical College; Wenzhou 325035; China
..............page:1459-1463
Cloning of bovine sox2 gene and construction of its retrovirus vector
Xiaoling Xin1; 2; Changrong Lü1; Dongmei Chen1; and Zhongying Dou1 1 Shaanxi Branch of National Stem Cell Engineering & Technology Center; Laboratory of Molecular Biology for Agriculture; College of Animal Medicine; Northwest A & F University; Yangling 712100; China 2 Institute of Animal Husbandry and Veterinary Medicine; Henan Academy of Agricultural Sciences; Zhengzhou 450002; China
..............page:1464-1469
Molecular and biochemical analysis of two genes encoding dehydroascorbate reductase in common wheat
Chunmei Yu1; Yanping Yang2; 3; Xinyan Liu1; Rong Zhou1; Liang Hua1; He Wei1; Shengjie Ding1; and Daowen Wang2 1 School of Life Sciences; Nantong University; Nantong 226007; China 2 Institute of Genetics and Developmental Biology; Chinese Academy of Science; Beijing 100101; China 3 Graduate University of Chinese Academy of Sciences; Beijing 100049; China
..............page:1483-1489
Role of nitric oxide and hydrogen peroxide in the essential oil increasing of suspension cells from Atractylodes lancea induced by endophytic fungal Cunninghamella sp. AL4 elicitor
Fang Fang1;2; Chuanchao Dai1; and Yu Wang1 1 College of Life Science; Nanjing Normal University; Nanjing 210046; China 2 College of Life Science and Chemical Engineering; Huaiyin Institute of Technology; Huai’an 223002; China
..............page:1490-1496
Tat transduction peptide displayed on the nucleocapsid improved the baculovirus transduction of mammalian cells
Li Li; Xin Wang; Juan Yin; and Jiang Zhong Department of Microbiology and Microbial Engineering; School of Life Sciences; Fudan University; Shanghai 200433; China
..............page:1558-1563
Expression, purification and characterization of HbsAg binding protein in Pichia pastoris
Yun Pang; Li Gong; Siyang Peng; and Naishuo Zhu School of Life Science; Institutes of Biomedical Science; Fudan University; Shanghai 200433; China
..............page:1564-1571
Discriminating acidic, neutral and alkaline enzymes with secondary structure amino acid composition
Guangya Zhang; Jiaqiang Gao; and Baishan Fang Department of Bioengineering and Biotechnology; Huaqiao University; Xiamen 361021; China
..............page:1508-1515
Adenovirus mediated IL-24 gene expression suppresses gastric cancer cell growth in vitro
Wanrong Bao; Jingcheng Miao; Weihua Sheng; Yunbo Shan; Zhengyi Li; Xiaohua Wang; Yingying Jing; Yali Han; and Jicheng Yang Department of Celluar and Molecular Biology; Medical School; Soochow University; Suzhou 215123; China
..............page:1586-1592
Immunogenicity and antigenicity of Japanese encephalitis virus envelope protein domain Ⅲ
Ying Huang; Shan Liu; Peng Yang; Yun Du; Zhiwei Sun; and Weiyuan Yu Laboratory for Protein Engineering; Institute of Biotechnology; Academy of Military Medical Sciences; Beijing 100071; China
..............page:1532-1537
Purification of anti-HBcAg monoclonal antibodies using immobilized metal ion affinity chromatography
Ji Zhu1; Yu Yi1; Yinfei Wu2; Keyin Zhu3; Jianfeng Mei1; Jianshu Chen1; and Guoqing Ying1 1 College of Pharmaceutical Science; Zhejiang University of Technology; Hangzhou 310014; China 2 ABON Biopharm Co.; Ltd; Hangzhou 310018; China 3 BIOLINK Biopharm Co.; Ltd; Hangzhou 310018; China
..............page:1572-1578
Optimized expression of the L1 protein of human papillomavirus in Hansenula polymorpha
Weiwei Li1;2; Xiuping He1; Xuena Guo1; Zhenying Zhang1; and Borun Zhang1 1 Laboratory of Yeast Molecular Genetics and Breeding; Institute of Microbiology; Chinese Academy of Sciences; Beijing 100101; China 2 Graduate University of Chinese Academy of Sciences; Beijing 100049; China
..............page:1516-1523
Over-expression of phospholipase D3 inhibits Akt phosphorylation in C2C12 myoblasts
Junlin Zhang1; Shuai Chen1; Shujin Zhang1; Zhijuan Lu1; Heping Yang2; and Huayan Wang1 1 Shaanxi Center of Stem Cell Engineering and Technology; Shaanxi Key Laboratory of Molecular Biology for Agriculture; College of Veterinary Medicine; Northwest A&F University; Yangling 712100; China 2 Yangling Vocational & Technical College; Department of Animal Engineering; Yangling 712100; China
..............page:1524-1531
A multipathogen selective enrichment broth (SVV) for simultaneous growth of Salmonella, Vibrio parahaemolyticus, and Vibrio cholerae
Yiying Qin; Hui Wu; Xinglong Xiao; Yigang Yu; Dongmei Liu; Xiaofeng Li; and Yuqian Tang College of Light Industry & Food; South China University of Technology; Guangzhou 510640; China
..............page:1497-1507
kuai xun
..............page:1469,1489,1496,1531,1551,1557
Construction and characterization of cardiac specific promoter-driven expression vector
Wenjun He*; Shichong Li*; Lingling Ye; Qiwei Wang; Haitao Wang; Jing Xie; Hong Liu; and Zhaolie Chen Department of Cell Engineering; Institute of Biotechnology; Academy of Military Medical Sciences; Beijing 100071; China
..............page:1546-1551
Construction of a recombinant adenovirus carrying the Hantaan virus glycoprotein G2 gene and its expression in Hela cells
Ting Huyan; Xiaoping Xue; Kai Song; Hua Wang; Hui Yang; and Wei Wang Faculty of Life Science; Northwestern Ploytechnical University; Xi’an 710072; China
..............page:1579-1585
Applications of reverse genetics in studying classical swine fever virus
Dafei Liu; Yuan Sun; and Huaji Qiu Division of Swine Infectious Diseases; State Key Laboratory of Veterinary Biotechnology; Harbin Veterinary Research Institute; Chinese Academy of Agricultural Sciences; Harbin 150001; China
..............page:1441-1448
Cloning and expression of fox growth hormone gene in Pichia pastoris
Wei Li1; Xiujin Li2; Fei Zhong1; Huijun Jin1; Min Xie1; Yuzhi Liu1; Longfei Liu1; and Qingjie Su1 1 Department of Basic Veterinary Medicine; College of Animal Science and Technology; Agricultural University of Hebei; Baoding 071001; China 2 Department of Biotechnology; College of Environmental and Chemical Engineering; Yanshan University; Qinhuangdao 066004; China
..............page:1470-1476
Biosorption of direct scarlet dye on magnetically modified Saccharomyces cerevisiae cells
Qi Wu; Zhi Shan; Mao Shen; Shuangjiang Li; and Hui Chen College of Life Science; Sichuan Agricultural University; Ya’an 625014; China
..............page:1477-1482