Progress in Veterinary Medicine 2013 Issue 12 Development of a Duplex Real-time PCR for Detection of H7N9 Avian Influenza Virus

Development of a Duplex Real-time PCR for Detection of H7N9 Avian Influenza Virus
CHEN Min-mei;,DENG Xian-wen;,FAN Qing;,LIU Jia-bo;,LUO Si-si;,PANG Yao-shan;,XIE Li-ji;,XIE Zhi-qin;,XIE Zhi-xun;,YANG Jin-ye;

..............page:1-5

Development and Application of RT-PCR for Detecting Avian Infectious Bursal Disease Virus
CAO Chen-fu;,CHEN Bing;,HUA Qun-yi;,LIAO Li-shan;,LIN Qing-yan;,LIU Jian-li;,Lü Jian-qiang;,LU Ti-kang;,QIN Zhi-feng;,RUAN Zhou-xi;,SUN Jie;,TAO Hong;,YANG Jun-xing;,ZENG Shao-ling;,ZHAN Ai-jun;,ZHANG Cai-hong;

..............page:6-10

Development of Real-time Fluorescent Quantitative RT-PCR for Detection of AHSV Based on SYBR GreenⅠ
LI Fu-xiang;,YANG Shi-biao;,ZHAO Wen-hua;

..............page:11-16

Cloning and Prokaryotic Expression of PRRSV GP5 Gene
MEI Yun-an;,WANG Lu-yan;,WANG Xiao-du;,YUAN Zhuang-chuan;,ZHANG Min;

..............page:17-21

Packaging of Lentivirus and Its Transgenic Efficiency in Poultry Cells
CHEN Zhi-sheng;,HUANG Xiao-min;,LIAO Jing-sen;

..............page:22-24

Improvement of Purification Method for Brucella Outer Membrane Protein 16
JIN Ya-ping;,WANG Ai-hua;,WANG Xiang-guo;,XU Qin;,YANG Lei;,ZHANG Jan-po;

..............page:25-28

Establishment and Application of Duplex PCR for Detection of Haemophilus parasuis and Actinobacillus pleuropneumoniae
YU Yuan-di;,YUE Hua;,ZENG Ze;,ZHANG Bin;

..............page:29-33

Preparation and Characterization of the Monoclonal Antibodies Against p27 Protein of Avian Leukosis Virus
CHENG Guo-fu;,GU Chang-qin;,HU Xue-ying;,LUO Qing-ping;,SHAO Hua-bin;,XIE Hua-li;,YANG Jun;,ZHANG Wan-po;

..............page:34-37

Cloning and Sequence Analysis of ALV gp85 Gene Subgroup from Two Chicken Farms J in Anhui
DAI Yin;,HOU Hong-yan;,HU Xiao-miao;,PAN Xiao-cheng;,ZHANG Dan-jun;,ZHAO Rui-hong;,ZHOU Xue-li;

..............page:38-41

Establishment and Application of a triplex PCR for Detection of SIV,PCV-2 and PRRSV
CHEN Sheng-feng;,CHEN Zhi-sheng;,JI Hui-qin;,WANG Bing-yu;,XU Zheng-na;

..............page:42-45

Resistance Change and Oxidative Stress-induced After Infecting Mice with Staphyloccocus aureus Isolated from Bovine
LI Hong-sheng;,LI Xin-pu;,LUO Jin-yin;,TIAN Yong-gang;,WANG Ling;,WANG Xu-rong;,YANG Feng;

..............page:46-50

Establishment of RT-PCR and Real-time RT-PCR Detection Method for Simian Retrovirus
HU Jian-hua;,HUANG Zhong-rong;,JIANG Jing;,LI Jian;,PAN Bao-jin;,WEI Xiao-feng;,XIONG Wei;,ZHANG Qiang;

..............page:51-54

Isolation and Sequence Analysis of Porcine Circovirus Type 2 in Chongqing Area
HOU Quan-shu;,LIANG Wang-wang;,LIN Lu;,LING Hong-quan;,SUN Yan;,WU Sheng-xi;,XIANG Fan;,ZENG Zheng;

..............page:55-59

Study on Transcriptional Regulation of rovA by OxyR inYersinia pestis
HAN Yan-ping;,HUANG Xin-xiang;,LIU Lei;,NI Bin;

..............page:60-63,64

Establishment of Standard Curve for mouse Bax Gene Recombinant Plasmid by Real-time qPCR
CAI Qing;,FU Wen-zhuo;,LU Qiang;,PAN Bo;,PAN Yao-qian;,WANG Ji-hong;

..............page:64-68

Establishment of Real-time RT-PCR for Detecting Rabbit Haemorrhagic Disease Virus
DING Zhuang;,SHEN Zhi-qiang;,XIAO Yue-qiang;,XIE Jin-wen;,XU Er-dan;

..............page:69-73

Effects of Specific IgY Antibodies Against PRRSV and PCV-2-Cap Protein on Growth Performance and Immunity of Weaning Piglets
BAO Wei-hua;,FAN Li;,FANG Wei-huan;,LI Xiao-liang;,LU Li-ming;,SUN Peng-lie;,ZHOU Xue-rong;

..............page:74-78,79

Cloning and Sequencing of NS Genes of Muscovy Duck Parvovirus ZQ Strain
FU Qiang;,SHEN Zhi-qiang;,WANG Wen-xiu;,ZHANG Song-lin;

..............page:79-83

Detection of FTO Gene in Different Tissues in Aletai Sheep and Little-tailed Han Sheep
WANG Jin-quan;,WANG Xiao-yan;,YAO Gang;,YE Qing;

..............page:84-88

Effects of Tibetan Kefir Fermented Milk on the Liver Mitochondrial Function in Aging Mice
CHENG Guang-dong;,L Dong-yun;,LI Chun-feng;,YUE Li-hong;,ZHANG Qiang;

..............page:89-92,93

Effects of Endotoxin on Mast Cell Counts of Immune Organs in Chicks
FENG Ding-guo;,OU De-yuan;,SHI Yun-gang;,WANG Qun-hui;,WU Yong-yin;,YAO Hong-yan;,YE Dong-guo;

..............page:93-96

Investigation on Several MHCⅠAlleles of Macaca mulatta in Southwestern of China
HE Zhan-long;,LI Yan-yan;,LIU long-ding;,LU Shuai-yao;,QI Su-dong;,YANG Feng-mei;,YU Wen-hai;,ZHAO Yuan;

..............page:97-100

Effects of Rapamycin on Early Development of Mouse In Vitro Fertilized Embryos
LI Yun-sheng;,WU Rong-hua;,ZHANG Xi-feng;,ZHANG Yu;,ZHANG Yun-hai;,ZHOU Liang;,ZHOU Na-ru;

..............page:101-105

Study on Effect of Compound Zhongjiefeng Particles on Eliminating Phlegm and Relieving Asthma
HE Jia-kang;,HU Ting-jun;,LAI Sheng-ji;,YANG Shan-zhong;

..............page:106-109

Histopathologic Observation and Blood Biochemical Index Analysis on the Diseased Cyprinus Carpio Haematopterus with Acute Gill-rot Disease
CAO Hai-peng;,GUO Guo-jun;,LI Li;,WANG Xian-ke;

..............page:110-114

Cloning and Sequence Analysis of SAG2 Gene in Eimeria tenella Changchun Strain
CAO Li-li;,HOU Hong-lie;,REN Ke-yan;,YAO Xin-hua;,YUAN Shu-xian;

..............page:115-119,120

Study on Methods for Preparation of Chromium-rich Milk
DU Dao-hui;,FENG Qian-jin;,LI Jin;

..............page:120-123

Expression of Estrogen Membrane Receptor GPR30 in Uterus of Pregnant Guanzhong Dairy Goats
DAI Ying-ying;,LIU Hong-gai;,QIN Chu-bin;,WANG Wen-li;,ZHAO Hui-ying;

..............page:124-127

Preparation and Quality Control of Compound Rhizoma dioscoreae hypoglaucae Granuales
GUO Lin-gang;,HAO Cai-ju;,LI Chao;,Zhao Chun-hong;,ZHAO De-lu;,ZUO Jian-wu;

..............page:128-130,131

Transplantation Effects of Induced Immortalized Pancreatic Mesenchymal Stem Cells in Diabetic Mice
FENG Jiu-xing;,LI Dan;,LI Qiang;,LIU Yu-xiao;,WANG Xue-qin;,ZHANG Hui-ru;

..............page:131-135

Study on Anti-fatigue and Hypoxia Tolerance Effects ofAstragalus Yiqi Granules
BAO Yu-long;,DU Jia-lin;,FAN Ying-lan;,LI Xian-hua;

..............page:136-138,139

Detection of Tissue Distribution of HSPA1A and HSPA2 Genes in Yaks
CHEN Ya-bing;,LAN Dao-liang;,LI Jiang;,LIN Bao-shan;,WANG Shu-mao;

..............page:139-144

Establishment and Application of PCR for Detection of Bacterial blaNDM-1 Gene
HUANG Zhong;,JIA Chun-ling;,L Dian-hong;,QIU Ting;,WEI Wen-kang;,WEN Xiao-hui;,YUAN Jie;,ZHAI Shao-lun;,ZHOU Xiu-rong;

..............page:145-148,149

Preparation and Quality Control of Veterinary Used Compound Policresulen Gel
LI Hui-fang;,LI Yin-qian;,LI Yuan;,LIU Dan;,LIU Yi-lin;,WANG Fang;

..............page:149-151

Progress on Innate Immune Responses Induced by Avian Infectious Bronchitis Virus
HE Yi-ning;,MO Mei-lan;,WEI Ping;,WEI Tian-chao;,XIE Zhi-wen;,ZHENG Yan-sheng;

..............page:152-155

Progress on Diagnosis Methods for Porcine Epidemic Diarrhea
WU Xu-jin;,XIONG Mang-li;,YIN Bao-ying;

..............page:156-159

Progress on Antiplasmid Effect of Chinese Herbal Medicine
KONG Tao;,WANG Guo-yong;,YANG Fan;,YANG Yu-rong;,ZHAO Zhen-sheng;

..............page:160-163,164

Progress on Pathogenicity and Mechanism of Campylobacter jejuni
GAI Wen-yan;,HUANG Xiu-mei;,QU Zhi-na;,WANG Juan;,WANG Jun-wei;,ZHAI Hai-hua;

..............page:164-169

Progress on Bacterial Efflux Pump Inhibitors of Plant Sources
LI Jing;,LIU Ming-chun;,TIAN Chun-lian;,ZHANG De-xian;,ZHU Xiao-ming;

..............page:170-172,173

Progress on Babesiosis
WU Jia-jun;,ZHOU Jin-lin;

..............page:173-178

Colloidal Gold Immunochromatographic Assay (GICA)for Detection of Veterinary Drug Residues in Livestock and Poultry Products
LIU li;,RONG Xia;,XUE Xiao;,YE Zi-xia;

..............page:179-182

Application of PCR-DGGE in Mammalian Gastrointestinal Microbial Diversity and Flora Structure Research
GAO Yun-hang;,GE Zheng;,MA Hong-xia;,ZHANG Fu-jun;,ZHAO Han-xu;

..............page:183-185

Risk Analysis on Transmission of Avian Influenza in Integrated Fish Farming
FU Xiao-zhe;,LAI Ying-tiao;,LI Ning-qiu;,REN Yan;,SHI Cun-bin;,WANG Ya-jun;,WU Shu-qin;

..............page:186-189

Technology for Superovulation and in Vitro Embryo Production in Lambs
WANG Li-min;,YIN Feng;

..............page:190-194

Progress on Chinese Herbal Medicine Against Avian Infectious Bronchitis
AI Wu;,HUANG Zhong-li;,LI Gui-ming;,LIN Shu-qian;,LIU Tao;,QI Li-hong;,SONG Min-xun;,YU Ke-xiang;

..............page:195-198

Advance in Molecular Mechanism of Infectious Bronchitis Virus Infection
LI Yu;,SHI Lei;,SUN Pei;,WANG Gui-jun;,WEI Jian-zhong;

..............page:199-203

Analyses of Development and Network Spread of Progress in Veterinary Medicine in Recent Years
CHEN Fang;,HUANG Jian-wen;,YI Lan;,ZHANG Yan-ming;

..............page:204-206

Distribution and Drug Sensitivity Test ofYersinia enterocolitica Between Dogs and Yaks in Partial Areas of Qinghai Province
CAO Hong-jun;,ZHANG Hong-jian;,ZHAO Jing;

..............page:207-212

Isolation,Identification and Drug Sensitivity Test of Proteus mirabilis from Bungarus multicinctus
GU Ying-le;,HUANG Yong;,LI Li;,XU Yong-li;,ZENG Yun;,ZHANG Yue-yun;,ZHAO Cheng-jian;

..............page:213-217

Immune Protective Efficiency of 4 Commercial Live Intermediate Virulence IBD Vaccines Against vvIBDV
HOU Hong-yan;,HU Xiao-miao;,ZHANG Dan-jun;,ZHAO Rui-hong;,ZHU Chuan-min;,ZHU Hui;

..............page:218-221

Investigation of Mastitis in Dairy Cows in Ningxia Area
DING Lin-jun;,HAN Kun;,HE Yu-long;,HU Deng-qiang;,LIU Jun-hong;,SHEN Ming-liang;,WANG Dong;,WANG Jian-bo;

..............page:222-227

Isolation,Identification and Drug Sensitivity Test of Clostridium perfringens
CHEN Hai-ning;,L Min-na;,LI Juan;,LIAO Shen-quan;,QI Nan-shan;,SUN Ming-fei;,WU Cai-yan;

..............page:228-231,232

Isolation and Identification of Aeromonas veronii from Fish
BIAN Yan-feng;,HU Xiu-cai;,L Ai-jun;,ZHAO La-mei;

..............page:232-235

Effects of Transport Stress on Dabie Mountain Yellow Cattle
CHEN Sheng;,LI Li-bing;,SU Shi-guang;,TANG Ji-shun;,WU Yong-gang;,XU Lei;

..............page:236-240

Passive Cutaneous Anaphylaxis Test in guinea pigs and Rats
LIU Yan-zhen;,SHAN Shu-xiang;,XIE Shu-juan;,YAN Jun;,ZHANG Dong-mei;,ZHU Chun-hua;

..............page:241-243

Detection and Efficacy Analysis of CSFV Antibodies from Pigs in Some Areas of Guangxi
HE Yi-jian;,LI Jun;,LIANG Sheng;,SU Jiao-xiu;,WEI Xian-kai;,YAN Jian-hua;,ZHENG Lie-feng;,ZHENG Min;,ZHONG Yi-zhi;

..............page:244-246

Histopathological Observation of Chicken Infectious Proventriculitis
WANG Jian-lin;,YIN Yan-bo;

..............page:247-250