1003-3505
2007 Issue 1
Generation of the Antibody Against the Schistosoma japonicum Antigen Sj22 with Genetic Immunization
peng hai-lin1 song kai2 ye sai2 song huai-guang2 liu feng3 xu bin4 zhang qing-hua 1; 2(1 state key laboratory of medical genomics; shanghai institute of hematology; rui jin hospital; shanghai jiao tong university school of medicine; shanghai 200025; china)(2 national engineering center for biochip at shanghai; shanghai 201203; china)(3 chinese national human genome center at shanghai; shanghai 201203; china)(4 institute of parasitic disease; chinese center for disease control and prevention; shanghai 200025; china)
peng hai-lin1 song kai2 ye sai2 song huai-guang2 liu feng3 xu bin4 zhang qing-hua 1; 2(1 state key laboratory of medical genomics; shanghai institute of hematology; rui jin hospital; shanghai jiao tong university school of medicine; shanghai 200025; china)(2 national engineering center for biochip at shanghai; shanghai 201203; china)(3 chinese national human genome center at shanghai; shanghai 201203; china)(4 institute of parasitic disease; chinese center for disease control and prevention; shanghai 200025; china)
..............page:6-10
Effect of Flow Cytometrically-sorted Sperm on Chromosomes of Bovine Blastocysts Produced in Vitro
zhang ming 1; 2 lu yang-qing 1; 2 lu ke-huan 1; 2(1 guangxi key laboratory for subtropical bio-resource conservation and utilization; nanning 530005; china)(2 animal reproduction institute; guangxi university; nanning 530005; china)
zhang ming 1; 2 lu yang-qing 1; 2 lu ke-huan 1; 2(1 guangxi key laboratory for subtropical bio-resource conservation and utilization; nanning 530005; china)(2 animal reproduction institute; guangxi university; nanning 530005; china)
..............page:98-101
Correction of a Mutation in a Synthetic Gene by DREAM Technique,a Site-directed Mutagenesis
zhu xiao-feng 1; 2 chen jin-hui 1; 3 zhang xin1 an xiao-ping1 zhou yu-sen1 tong yi-gang1(1 state key laboratory of pathogen and biosecurity; institute of microbiology and epidemiology; academy of military medical science; beijing 100071; china)(2 hospital no 451; pla; xi an 710054; china 3 shenyang agriculture university; shenyang 110161; china)
zhu xiao-feng 1; 2 chen jin-hui 1; 3 zhang xin1 an xiao-ping1 zhou yu-sen1 tong yi-gang1(1 state key laboratory of pathogen and biosecurity; institute of microbiology and epidemiology; academy of military medical science; beijing 100071; china)(2 hospital no 451; pla; xi an 710054; china 3 shenyang agriculture university; shenyang 110161; china)
..............page:86-92
Preparation and Characteristic of Monoclonal Antibodies to Tag of FLAG and Its Primary Application
min yu-tao 1; 2 wang yun-long 1* li chen-yang1 li yu-lin1 wang zhen3(1 henan biotechnology research center; zhengzhou 450002; china)(2 zhengzhou university of light industry; zhengzhou 450002; china)(3 zhengzhou technical college; zhengzhou 450121; china)
min yu-tao 1; 2 wang yun-long 1* li chen-yang1 li yu-lin1 wang zhen3(1 henan biotechnology research center; zhengzhou 450002; china)(2 zhengzhou university of light industry; zhengzhou 450002; china)(3 zhengzhou technical college; zhengzhou 450121; china)
..............page:93-97
Construction of OTX1 Lentiviral Vector and Overexpression Research
ren ping wang shu-yan guan yun-qian xu yan-ling zhang yu(cell therapy center; beijing institute of geriatrics; xuanwu hospital; capital university of medical science; beijing 100053; china)
ren ping wang shu-yan guan yun-qian xu yan-ling zhang yu(cell therapy center; beijing institute of geriatrics; xuanwu hospital; capital university of medical science; beijing 100053; china)
..............page:16-21
Agrobacterium-mediated Transformation of Tall Fescue and Perennial Ryegrass
wang yan-li1 ye xing-guo1 dong fang2 qiao wei-hua3 tao li-li1 li xiao-lu4 xu xing5(1 key laboratory for crop genetics and breeding of agricultural ministry institute of crop breeding and cultivation chinese academy of agricultural sciences; beijing 100081; china)(2 agricultural college ningxia university; yinchuan 750021; china)(3 college for biotechnology shangdong agricultural university; taian 271018; china)(4 the college of chemistry and environment engineering beijing technology and business university; beijing 100037; china)(5 ningxia key lab for agricultural biotechnology; yinchuan 750002; china)
wang yan-li1 ye xing-guo1 dong fang2 qiao wei-hua3 tao li-li1 li xiao-lu4 xu xing5(1 key laboratory for crop genetics and breeding of agricultural ministry institute of crop breeding and cultivation chinese academy of agricultural sciences; beijing 100081; china)(2 agricultural college ningxia university; yinchuan 750021; china)(3 college for biotechnology shangdong agricultural university; taian 271018; china)(4 the college of chemistry and environment engineering beijing technology and business university; beijing 100037; china)(5 ningxia key lab for agricultural biotechnology; yinchuan 750002; china)
..............page:22-27
Recombinant Baculoviruses as Mammalian Cell Gene-delivery Vectors:a Review
peng wu-ping qiu hua-ji(national key laboratory of veterinary biotechnology; harbin veterinary research institute; chinese academy of agricultural sciences; harbin 150001; china)
peng wu-ping qiu hua-ji(national key laboratory of veterinary biotechnology; harbin veterinary research institute; chinese academy of agricultural sciences; harbin 150001; china)
..............page:126-130
Effect of Overexpression of NAD+-dependent Malic Enzyme on Anaerobic Mixed Acid Fermentation of E.coli FMJ39
jiang min xie xin xu lin yan ming(college of life science and pharmaceutical engineering; nanjing university of technology; nanjing 210009; china)
jiang min xie xin xu lin yan ming(college of life science and pharmaceutical engineering; nanjing university of technology; nanjing 210009; china)
..............page:69-74
Disruption of hom Gene Encoding for Homoserine Dehydrogenase of Corynebacterium glutamicum
rao zhi-ming zhang jun-sheng shen wei fang hui-ying zhuge jian(research centre of industrial microorganisms and the key lab of industrial biotechnology; ministry of education; southern yangtze university; wuxi 214036; china)
rao zhi-ming zhang jun-sheng shen wei fang hui-ying zhuge jian(research centre of industrial microorganisms and the key lab of industrial biotechnology; ministry of education; southern yangtze university; wuxi 214036; china)
..............page:59-63
..............page:138-139
Research on Antimicrobial Peptides and Their Function
xu ling-long 1; 2 wang yun-feng 1; 2 shi xing-ming2 tong guang-zhi2(1 the college of life science; northeast agricultural university; harbin 150030; china)(2 division of avian infectious diseases; national key laboratory of veterinary biotechnology; harbin veterinary research institute chinese academy of agricultural science; harbin 150001; china)
xu ling-long 1; 2 wang yun-feng 1; 2 shi xing-ming2 tong guang-zhi2(1 the college of life science; northeast agricultural university; harbin 150030; china)(2 division of avian infectious diseases; national key laboratory of veterinary biotechnology; harbin veterinary research institute chinese academy of agricultural science; harbin 150001; china)
..............page:115-118
Ferment Engineering and Validation Ideas
liu ming-li(the qa department of genescience pharmaceuticals co.; ltd.; chingchun 130012; china)
liu ming-li(the qa department of genescience pharmaceuticals co.; ltd.; chingchun 130012; china)
..............page:131-136
..............page:140
Research Progress on Hepatitis C Virus Multi-epitope DNA Vaccine
yang hua-qiang cao yi-cheng(school of bioscience and bioengineering; south china university of technology; guangzhou 510641; china)
yang hua-qiang cao yi-cheng(school of bioscience and bioengineering; south china university of technology; guangzhou 510641; china)
..............page:119-125
High Level Prokaryotic Expression of Thymosin α1 by Gene Repeats
jin ming-fei1 xu wei-dong2 huang jing1 jin li1 wang jia1 ye hai-feng1 wu zi-rong1(1 school of life science; east china normal university; shanghai 200062; china)(2 shanghai institute for drug control; shanghai 200233; china)
jin ming-fei1 xu wei-dong2 huang jing1 jin li1 wang jia1 ye hai-feng1 wu zi-rong1(1 school of life science; east china normal university; shanghai 200062; china)(2 shanghai institute for drug control; shanghai 200233; china)
..............page:11-15
Clone and Sequence Analysis of Antheraea pernyi Nucleopolyhedrovirus PstI-B and C fragments
shi sheng-lin 1; 2 pan min-hui1 lu cheng 1**(1 key sericultural laboratory of agriculture ministry; southwest university; chongqing 400716; china)(2 college of bioscience and biotechnology; shenyang agricultural university; shenyang 110161; china)
shi sheng-lin 1; 2 pan min-hui1 lu cheng 1**(1 key sericultural laboratory of agriculture ministry; southwest university; chongqing 400716; china)(2 college of bioscience and biotechnology; shenyang agricultural university; shenyang 110161; china)
..............page:81-85
Selection of Liquid Media for High-density Fermentation of Recombinant E.coli for Production of Plasmids
zhang quan1 guo xiao-yu2 yuan wei-feng1 sun huai-chang1 zhu hong-fei 2(1 collge of veteriniary medicine yangzhou university; yangzhou 225009; china)(2 institute of animal science and veterinary medicine chinese academy of agricultural science; beijing 100081; china)
zhang quan1 guo xiao-yu2 yuan wei-feng1 sun huai-chang1 zhu hong-fei 2(1 collge of veteriniary medicine yangzhou university; yangzhou 225009; china)(2 institute of animal science and veterinary medicine chinese academy of agricultural science; beijing 100081; china)
..............page:102-105
The Study and Design Methods of Serum-Free Medium for Animal Cells
wang yong-min chen zhao-lie(institute of biotechnology; academy of military medical science; beijing 100071; china)
wang yong-min chen zhao-lie(institute of biotechnology; academy of military medical science; beijing 100071; china)
..............page:110-114
..............page:137-138
Exogenous Alanine Enhances the Expression of Spidroin2 cDNA of Spider Dragline Silk Protein in Escherichia coli
ma he-wen1 zhang li-shu2 zheng wei3 zhang yong-liang1 zhang yu-jing1(1 college of animal science and veterinary medicine; jilin university; changchun 130062; china)(2 the 11th institute; academy of military medical sciences; changchun 130062; china)(3 the 9th institute; academy of military medical sciences; beijing 100850; china)
ma he-wen1 zhang li-shu2 zheng wei3 zhang yong-liang1 zhang yu-jing1(1 college of animal science and veterinary medicine; jilin university; changchun 130062; china)(2 the 11th institute; academy of military medical sciences; changchun 130062; china)(3 the 9th institute; academy of military medical sciences; beijing 100850; china)
..............page:47-51
A Secretive Pichia pastoris Expression Vector for Direct PCR Product Cloning
zhang chao liu gang yu shao-wen xing miao(shenzhen key laboratory of microbial genetic engineering; college of life sciences; shenzhen university; shenzhen 518060; china)
zhang chao liu gang yu shao-wen xing miao(shenzhen key laboratory of microbial genetic engineering; college of life sciences; shenzhen university; shenzhen 518060; china)
..............page:52-58
Construction of SSR Genetic Map and Its QTL Analysis of Agronomic Traits with Sequenced Varieties
liang yong-shu 1; 2; 3 zhang qi-jun 1; 2 wang shi-quan 1; 2 deng qi-ming 1; 2 li ping 1; 2 li yan-ping3 yan shuang-yong3(1 rice research institute of sichuan agricultural university; wenjiang 611130; china)(2 key laboratory of southwest crop genetic resources and improvement ministry of education; sichuan agricultural university; ya an 625014; china)(3 institute of crops; academy of agricultural sciences; tianjin 300112; china)
liang yong-shu 1; 2; 3 zhang qi-jun 1; 2 wang shi-quan 1; 2 deng qi-ming 1; 2 li ping 1; 2 li yan-ping3 yan shuang-yong3(1 rice research institute of sichuan agricultural university; wenjiang 611130; china)(2 key laboratory of southwest crop genetic resources and improvement ministry of education; sichuan agricultural university; ya an 625014; china)(3 institute of crops; academy of agricultural sciences; tianjin 300112; china)
..............page:28-34
Gene Cloning and Eukaryotic Expression Vector Construction of Porcine lnterleukin-18
zheng lan-lan 1; 2 cui bao-an 1; 2 chen rui-liang1 jia yun-fei1 chen hong-ying 1; 2 fang zhong-yi 1.2 li xiao-kang 1 2 zhao li1(1 the college of animal husbandry and veterinary; henan agricultural university; zhengzhou 450002; china)(2 henan animal food safety key laboratory; zhengzhou 450002; china)
zheng lan-lan 1; 2 cui bao-an 1; 2 chen rui-liang1 jia yun-fei1 chen hong-ying 1; 2 fang zhong-yi 1.2 li xiao-kang 1 2 zhao li1(1 the college of animal husbandry and veterinary; henan agricultural university; zhengzhou 450002; china)(2 henan animal food safety key laboratory; zhengzhou 450002; china)
..............page:41-46
Construction and Identification of Recombinant Adenovirus Coexpression PRRSV GP5 Gene and Porcine Interferonγ Gene
niu ming-fu1 li xiang2 cao rui-bing1 zheng qi-sheng1 wei jian-chao1 ji xiang-bo1 li peng1 zhou bin1 chen pu-yan1(1 key laboratory of animal diseases diagnostic and immunology; ministry of agriculture; nanjing agriculture university; nanjing 210095; china)(2 department of base medical college of xinxiang medical college; xinxiang 453003; china)
niu ming-fu1 li xiang2 cao rui-bing1 zheng qi-sheng1 wei jian-chao1 ji xiang-bo1 li peng1 zhou bin1 chen pu-yan1(1 key laboratory of animal diseases diagnostic and immunology; ministry of agriculture; nanjing agriculture university; nanjing 210095; china)(2 department of base medical college of xinxiang medical college; xinxiang 453003; china)
..............page:35-40
Studies on Bacteriostatic Effect of Honggumycin
xiong zhi-qiang 1; 2 zhang si-liang1 tu guo-quan2(1 state key laboratory of bioreactor engineering ecust national engineering research center of biotechnology; shanghai 200237; china 2 department of bioengineering jau; nanchang 330045; china)
xiong zhi-qiang 1; 2 zhang si-liang1 tu guo-quan2(1 state key laboratory of bioreactor engineering ecust national engineering research center of biotechnology; shanghai 200237; china 2 department of bioengineering jau; nanchang 330045; china)
..............page:106-109
Effect of Culture Conditions on Jarosite for Immobilized Acidothiobacillus ferrooxidans
yang xiao-juan1 wang yu-jian 1; 2 li hong-yu 1* tu wei1(1 college of life science lanzhou university; lanzhou 730000; china)(2 school of chemical and biological engineering lanzhou jiaotong university; lanzhou 730070; china)
yang xiao-juan1 wang yu-jian 1; 2 li hong-yu 1* tu wei1(1 college of life science lanzhou university; lanzhou 730000; china)(2 school of chemical and biological engineering lanzhou jiaotong university; lanzhou 730070; china)
..............page:64-68
Purification of Trypsin by Using AOT/Isooctane Reverse Micellar Extraction and Overcoming Denaturation and Precipitation in the Process
chen lin-jie cao xue-jun(state key laboratory of bioreactor engineering; bioengineering institute; east china university of science and technology; shanghai 200237; china)
chen lin-jie cao xue-jun(state key laboratory of bioreactor engineering; bioengineering institute; east china university of science and technology; shanghai 200237; china)
..............page:75-80
Activity Study of Ciliary Neurotrophic Factor(CNTF)Mutant
bi hua yuan li-yong shi xin-chang zhao yang liu lan rao chun-ming wang jun-zhi(department of biochemistry; national institute for the control of pharmaceutical and biological products; beijing 100050; china)
bi hua yuan li-yong shi xin-chang zhao yang liu lan rao chun-ming wang jun-zhi(department of biochemistry; national institute for the control of pharmaceutical and biological products; beijing 100050; china)
..............page:1-5